Paper-based synthetic gene networks

Keith Pardee, Alexander Green, Tom Ferrante, D. Ewen Cameron, Ajay Daleykeyser, Peng Yin, James J. Collins

Research output: Contribution to journalArticle

217 Citations (Scopus)

Abstract

Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.

Original languageEnglish (US)
Pages (from-to)940-954
Number of pages15
JournalCell
Volume159
Issue number4
DOIs
StatePublished - Nov 6 2014
Externally publishedYes

Fingerprint

Synthetic Genes
Gene Regulatory Networks
Genes
Networks (circuits)
Ebolavirus
Synthetic Biology
Glucose sensors
Technology
Cell-Free System
Rapid prototyping
Viruses
Industry
Education
Switches
Health
RNA
Costs and Cost Analysis
Glucose
Molecules
Sensors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pardee, K., Green, A., Ferrante, T., Cameron, D. E., Daleykeyser, A., Yin, P., & Collins, J. J. (2014). Paper-based synthetic gene networks. Cell, 159(4), 940-954. https://doi.org/10.1016/j.cell.2014.10.004

Paper-based synthetic gene networks. / Pardee, Keith; Green, Alexander; Ferrante, Tom; Cameron, D. Ewen; Daleykeyser, Ajay; Yin, Peng; Collins, James J.

In: Cell, Vol. 159, No. 4, 06.11.2014, p. 940-954.

Research output: Contribution to journalArticle

Pardee, K, Green, A, Ferrante, T, Cameron, DE, Daleykeyser, A, Yin, P & Collins, JJ 2014, 'Paper-based synthetic gene networks', Cell, vol. 159, no. 4, pp. 940-954. https://doi.org/10.1016/j.cell.2014.10.004
Pardee K, Green A, Ferrante T, Cameron DE, Daleykeyser A, Yin P et al. Paper-based synthetic gene networks. Cell. 2014 Nov 6;159(4):940-954. https://doi.org/10.1016/j.cell.2014.10.004
Pardee, Keith ; Green, Alexander ; Ferrante, Tom ; Cameron, D. Ewen ; Daleykeyser, Ajay ; Yin, Peng ; Collins, James J. / Paper-based synthetic gene networks. In: Cell. 2014 ; Vol. 159, No. 4. pp. 940-954.
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