TY - JOUR
T1 - Paper-based synthetic gene networks
AU - Pardee, Keith
AU - Green, Alexander A.
AU - Ferrante, Tom
AU - Cameron, D. Ewen
AU - Daleykeyser, Ajay
AU - Yin, Peng
AU - Collins, James J.
N1 - Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2014/11/6
Y1 - 2014/11/6
N2 - Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.
AB - Synthetic gene networks have wide-ranging uses in reprogramming and rewiring organisms. To date, there has not been a way to harness the vast potential of these networks beyond the constraints of a laboratory or in vivo environment. Here, we present an in vitro paper-based platform that provides an alternate, versatile venue for synthetic biologists to operate and a much-needed medium for the safe deployment of engineered gene circuits beyond the lab. Commercially available cell-free systems are freeze dried onto paper, enabling the inexpensive, sterile, and abiotic distribution of synthetic-biology-based technologies for the clinic, global health, industry, research, and education. For field use, we create circuits with colorimetric outputs for detection by eye and fabricate a low-cost, electronic optical interface. We demonstrate this technology with small-molecule and RNA actuation of genetic switches, rapid prototyping of complex gene circuits, and programmable in vitro diagnostics, including glucose sensors and strain-specific Ebola virus sensors.
UR - http://www.scopus.com/inward/record.url?scp=84909963313&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84909963313&partnerID=8YFLogxK
U2 - 10.1016/j.cell.2014.10.004
DO - 10.1016/j.cell.2014.10.004
M3 - Article
C2 - 25417167
AN - SCOPUS:84909963313
SN - 0092-8674
VL - 159
SP - 940
EP - 954
JO - Cell
JF - Cell
IS - 4
ER -