Pancreatic acinar cells: effects of lanthanum ions on amylase release and calcium ion fluxes

D. E. Chandler, J. A. Williams

Research output: Contribution to journalArticle

Abstract

The effect of La3+ on amylase release and Ca2+ fluxes in mouse pancreatic fragments in vitro was studied. Amylase release was increased by 0.1 mM La3+ and progressively inhibited by 1.0-10 mM La3+. Non stimulated and bethanecol stimulated secretion were altered in an identical manner. Inhibition of amylase release was rapid and reversible. Uptake of 45Ca2+ was multiphasic with equilibrium with stable Ca2+ still not complete after 2 hr. La3+ (10 mM) limited uptake of 45Ca2+ to the extracellular space and slightly decreased total Ca2+ content. Lower concentrations of La3+ affected 45Ca2+ uptake and total Ca2+ content in a biphasic manner which paralleled effects on amylase release. La3+ restricted washout of 45Ca2+ to isotope in the extracellular space and abolished the bethanecol stimulated increase in 45Ca2+ efflux. Uptake of 45Ca2+ into intracellular space, as measured by the 'lanthanum' method, was not affected by bethanecol. Tissue ultrastructure and Na+ and K+ content were not affected by La3+. It is concluded that an influx of extracellular Ca2+ is not important for triggering of secretion and that La3+ may inhibit amylase release by acting on the release process rather than on Ca2+ influx.

Original languageEnglish (US)
Pages (from-to)831-846
Number of pages16
JournalJournal of Physiology
Volume243
Issue number3
StatePublished - 1974
Externally publishedYes

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'Pancreatic acinar cells: effects of lanthanum ions on amylase release and calcium ion fluxes'. Together they form a unique fingerprint.

  • Cite this