Pan-Nematoda Transcriptomic Elucidation of Essential Intestinal Functions and Therapeutic Targets With Broad Potential

Qi Wang, Bruce A. Rosa, Douglas P. Jasmer, Makedonka Mitreva

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The nematode intestine is continuous with the outside environment, making it easily accessible to anthelmintics for parasite control, but the development of new therapeutics is impeded by limited knowledge of nematode intestinal cell biology. We established the most comprehensive nematode intestinal functional database to date by generating transcriptional data from the dissected intestines of three parasitic nematodes spanning the phylum, and integrating the results with the whole proteomes of 10 nematodes (including 9 pathogens of humans or animals) and 3 host species and 2 outgroup species. We resolved 10,772 predicted nematode intestinal protein families (IntFams), and studied their presence and absence within the different lineages (births and deaths) among nematodes. Conserved intestinal cell functions representing ancestral functions of evolutionary importance were delineated, and molecular features useful for selective therapeutic targeting were identified. Molecular patterns conserved among IntFam proteins demonstrated large potential as therapeutic targets to inhibit intestinal cell functions with broad applications towards treatment and control of parasitic nematodes.

Original languageEnglish (US)
Pages (from-to)1079-1089
Number of pages11
JournalEBioMedicine
Volume2
Issue number9
DOIs
StatePublished - Sep 1 2015

Keywords

  • Intestinal protein families
  • Molecular functions
  • Nematode intestine
  • Therapeutic targets

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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