Pan-enterovirus amplicon-based high-throughput sequencing detects the complete capsid of a eva71 genotype c1 variant via wastewater-based epidemiology in Arizona

Temitope O.C. Faleye; Erin Driver; Devin Bowes; Sangeet Adhikari; Deborah Adams; Arvind Varsani; Rolf U. Halden; Matthew Scotch

doi:10.3390/v13010074

Pan-enterovirus amplicon-based high-throughput sequencing detects the complete capsid of a eva71 genotype c1 variant via wastewater-based epidemiology in Arizona

Temitope O.C. Faleye, Erin Driver, Devin Bowes, Sangeet Adhikari, Deborah Adams, Arvind Varsani, Rolf U. Halden, Matthew Scotch

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have been circulating for over two years, but its sequence has not been documented in that period. As the SARS-CoV-2 pandemic has resulted in changes in health-seeking behavior and overwhelmed pathogen diagnostics, our findings highlight the importance of wastewater-based epidemiology (WBE ) as an early warning system for virus surveillance.

Original language	English (US)
Article number	74
Journal	Viruses
Volume	13
Issue number	1
DOIs	https://doi.org/10.3390/v13010074
State	Published - Jan 2021

Keywords

Arizona
Enterovirus A
Human
Molecular epidemiology
Poliomyelitis
Wastewater

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v13010074

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title = "Pan-enterovirus amplicon-based high-throughput sequencing detects the complete capsid of a eva71 genotype c1 variant via wastewater-based epidemiology in Arizona",

abstract = "We describe the complete capsid of a genotype C1-like Enterovirus A71 variant recovered from wastewater in a neighborhood in the greater Tempe, Arizona area (Southwest United States) in May 2020 using a pan-enterovirus amplicon-based high-throughput sequencing strategy. The variant seems to have been circulating for over two years, but its sequence has not been documented in that period. As the SARS-CoV-2 pandemic has resulted in changes in health-seeking behavior and overwhelmed pathogen diagnostics, our findings highlight the importance of wastewater-based epidemiology (WBE ) as an early warning system for virus surveillance.",

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AU - Bowes, Devin

AU - Adhikari, Sangeet

AU - Adams, Deborah

AU - Varsani, Arvind

AU - Halden, Rolf U.

AU - Scotch, Matthew

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Pan-enterovirus amplicon-based high-throughput sequencing detects the complete capsid of a eva71 genotype c1 variant via wastewater-based epidemiology in Arizona

Abstract

Keywords

ASJC Scopus subject areas

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