PAK1 kinase is required for CXCL1-induced chemotaxis

Dingzhi Wang, Jiging Sai, Glendora Carter, Aristidis Sachpatzidis, Elias Lolis, Ann Richmond

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31 Scopus citations

Abstract

The CXC subfamily of chemokines plays an important role in diverse processes, including inflammation, wound healing, growth regulation, angiogenesis, and tumorigenesis. The CXC chemokine CXCL1, or MGSA/GROα, is traditionally considered to be responsible for attracting leukocytes into sites of inflammation. To better understand the molecular mechanisms by which CXCL1 induces CXCR2-mediated chemotaxis, the signal transduction components involved in CXCL1-induced chemotaxis were examined. It is shown here that CXCL1 induces cdc42 and PAK1 activation in CXCR2-expressing HEK293 cells. Activation of the cdc42-PAK1 cascade is required for CXCL1-induced chemotaxis but not for CXCL1-induced intracellular Ca2+ mobilization. Moreover, CXCL1 activation of PAK1 is independent of ERK1/2 activation, a conclusion based on the observations that the inhibition of MEK-ERK activation by expression of dominant negative ERK or by the MEK inhibitor, PD98059, has no effect on CXCL1-induced PAK1 activation or CXCL1-induced chemotaxis.

Original languageEnglish (US)
Pages (from-to)7100-7107
Number of pages8
JournalBiochemistry
Volume41
Issue number22
DOIs
StatePublished - Jun 4 2002

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Wang, D., Sai, J., Carter, G., Sachpatzidis, A., Lolis, E., & Richmond, A. (2002). PAK1 kinase is required for CXCL1-induced chemotaxis. Biochemistry, 41(22), 7100-7107. https://doi.org/10.1021/bi025902m