Oxygen transfer from bleomycin-metal complexes

N. Murugesan, G. M. Ehrenfeld, Sidney Hecht

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Both Fe(III) and Cu(II) complexes of bleomycin (BLM), but not N-acetyl BLM · Fe(III), mediated the transfer of oxygen from iodosobenzene to organic substrates. In analogy with results obtained using certain cytochrome P-450 analogs, cis-stilbene was converted cleanly to the respective oxide, while no more than traces of trans-stilbene oxide were formed from trans-stilbene under identical conditions. The possible relevance of these observations to the degradation of DNA by bleomycin was also studied. In both the presence and absence of O2, BLM · Cu(II) · C6H5IO effected DNA degradation, as judged by the release of [3H]thymine from radiolabeled Escherichia coli DNA. These findings provide a valuable new assay system for the study of bleomycin analogs and suggest the possibility that bleomycin may function as an 'oxygen transferase' in its degradation of DNA in situ.

Original languageEnglish (US)
Pages (from-to)8600-8603
Number of pages4
JournalJournal of Biological Chemistry
Volume257
Issue number15
StatePublished - 1982
Externally publishedYes

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Coordination Complexes
Bleomycin
Oxygen
Stilbenes
DNA
Degradation
Thymine
Transferases
Cytochrome P-450 Enzyme System
Oxides
Escherichia coli
Assays
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Murugesan, N., Ehrenfeld, G. M., & Hecht, S. (1982). Oxygen transfer from bleomycin-metal complexes. Journal of Biological Chemistry, 257(15), 8600-8603.

Oxygen transfer from bleomycin-metal complexes. / Murugesan, N.; Ehrenfeld, G. M.; Hecht, Sidney.

In: Journal of Biological Chemistry, Vol. 257, No. 15, 1982, p. 8600-8603.

Research output: Contribution to journalArticle

Murugesan, N, Ehrenfeld, GM & Hecht, S 1982, 'Oxygen transfer from bleomycin-metal complexes', Journal of Biological Chemistry, vol. 257, no. 15, pp. 8600-8603.
Murugesan, N. ; Ehrenfeld, G. M. ; Hecht, Sidney. / Oxygen transfer from bleomycin-metal complexes. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 15. pp. 8600-8603.
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