TY - GEN
T1 - Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope
AU - Chao, Shih Hui
AU - Holl, Mark R.
AU - McQuaide, Sarah C.
AU - Meldrum, Deirdre R.
PY - 2006
Y1 - 2006
N2 - A digital light modulation microscope (DLMM) using a digital micro-mirror device (DMD, Texas Instruments) has been developed to enable detection of O 2 concentration in micro-bioreactors using O2-quenching porphyrin phosphorescent dyes. The emission intensity and phosphorescence lifetime of such dyes are both a function of O2 concentration. While emission intensity can vary in these dye systems as a function of concentration and illumination intensity, phosphorescence lifetime is primarily sensitive to only O2 concentration. In contrast to conventional phosphorescence lifetime imaging, the DLMM eliminates the need for a pulsed light source, scanning mirrors, or a high-speed camera for time-gated imaging. This technique can selectively address structured light illumination to each sensor location, which is a beneficial feature for analysis of large micro-sensor arrays within lab-on-a-chip devices. The mirrors on the DMD perform as electronically addressable optical switches, each having a ∼15 μs switching time, shorter than the phosphorescence lifetimes of potential O2 sensing dyes (∼25-100 μs). The structured light pattern of the DMD and the switching rate of the mirrors are controlled by a PC. An arc lamp illuminates the DMD uniformly and then projects to the specimen through a filter cube for the selected phosphorescent sensor compound. The emitted light returns to the filter cube and is detected by a photo multiplier tube (PMT). An oscilloscope is used to record the emission signal waveform from the PMT. To demonstrate O 2 sensing with lab-on-a-chip devices, an array of 150-μmdiameter micro-wells coated with phosphorescent porphyrin were observed using the DLMM. The goal of this platform is to measure the O2 consumption of individual cells trapped in the microwells.
AB - A digital light modulation microscope (DLMM) using a digital micro-mirror device (DMD, Texas Instruments) has been developed to enable detection of O 2 concentration in micro-bioreactors using O2-quenching porphyrin phosphorescent dyes. The emission intensity and phosphorescence lifetime of such dyes are both a function of O2 concentration. While emission intensity can vary in these dye systems as a function of concentration and illumination intensity, phosphorescence lifetime is primarily sensitive to only O2 concentration. In contrast to conventional phosphorescence lifetime imaging, the DLMM eliminates the need for a pulsed light source, scanning mirrors, or a high-speed camera for time-gated imaging. This technique can selectively address structured light illumination to each sensor location, which is a beneficial feature for analysis of large micro-sensor arrays within lab-on-a-chip devices. The mirrors on the DMD perform as electronically addressable optical switches, each having a ∼15 μs switching time, shorter than the phosphorescence lifetimes of potential O2 sensing dyes (∼25-100 μs). The structured light pattern of the DMD and the switching rate of the mirrors are controlled by a PC. An arc lamp illuminates the DMD uniformly and then projects to the specimen through a filter cube for the selected phosphorescent sensor compound. The emitted light returns to the filter cube and is detected by a photo multiplier tube (PMT). An oscilloscope is used to record the emission signal waveform from the PMT. To demonstrate O 2 sensing with lab-on-a-chip devices, an array of 150-μmdiameter micro-wells coated with phosphorescent porphyrin were observed using the DLMM. The goal of this platform is to measure the O2 consumption of individual cells trapped in the microwells.
KW - Digital light modulation microscope
KW - Digital micro-mirror device
KW - High content screening
KW - Lab-on-a-chip
KW - Oxygen concentration measurement
KW - Phosphorescence lifetime detection
KW - Single cell analysis
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U2 - 10.1117/12.644939
DO - 10.1117/12.644939
M3 - Conference contribution
AN - SCOPUS:33646181224
SN - 081946130X
SN - 9780819461308
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Y2 - 23 January 2006 through 25 January 2006
ER -