Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope

Shih-Hui Chao, Mark R. Holl, Sarah C. McQuaide, Deirdre Meldrum

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

A digital light modulation microscope (DLMM) using a digital micro-mirror device (DMD, Texas Instruments) has been developed to enable detection of O 2 concentration in micro-bioreactors using O2-quenching porphyrin phosphorescent dyes. The emission intensity and phosphorescence lifetime of such dyes are both a function of O2 concentration. While emission intensity can vary in these dye systems as a function of concentration and illumination intensity, phosphorescence lifetime is primarily sensitive to only O2 concentration. In contrast to conventional phosphorescence lifetime imaging, the DLMM eliminates the need for a pulsed light source, scanning mirrors, or a high-speed camera for time-gated imaging. This technique can selectively address structured light illumination to each sensor location, which is a beneficial feature for analysis of large micro-sensor arrays within lab-on-a-chip devices. The mirrors on the DMD perform as electronically addressable optical switches, each having a ∼15 μs switching time, shorter than the phosphorescence lifetimes of potential O2 sensing dyes (∼25-100 μs). The structured light pattern of the DMD and the switching rate of the mirrors are controlled by a PC. An arc lamp illuminates the DMD uniformly and then projects to the specimen through a filter cube for the selected phosphorescent sensor compound. The emitted light returns to the filter cube and is detected by a photo multiplier tube (PMT). An oscilloscope is used to record the emission signal waveform from the PMT. To demonstrate O 2 sensing with lab-on-a-chip devices, an array of 150-μmdiameter micro-wells coated with phosphorescent porphyrin were observed using the DLMM. The goal of this platform is to measure the O2 consumption of individual cells trapped in the microwells.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6088
DOIs
StatePublished - 2006
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV - San Jose, CA, United States
Duration: Jan 23 2006Jan 25 2006

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
CountryUnited States
CitySan Jose, CA
Period1/23/061/25/06

Fingerprint

Phosphorescence
Light modulation
Sensor arrays
Microscopes
Dyes
Lab-on-a-chip
Oxygen
Porphyrins
Lighting
Arc lamps
Imaging techniques
Optical switches
High speed cameras
Sensors
Bioreactors
Light sources
Quenching
Mirrors
Scanning

Keywords

  • Digital light modulation microscope
  • Digital micro-mirror device
  • High content screening
  • Lab-on-a-chip
  • Oxygen concentration measurement
  • Phosphorescence lifetime detection
  • Single cell analysis

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Chao, S-H., Holl, M. R., McQuaide, S. C., & Meldrum, D. (2006). Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 6088). [60880S] https://doi.org/10.1117/12.644939

Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope. / Chao, Shih-Hui; Holl, Mark R.; McQuaide, Sarah C.; Meldrum, Deirdre.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088 2006. 60880S.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chao, S-H, Holl, MR, McQuaide, SC & Meldrum, D 2006, Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 6088, 60880S, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, San Jose, CA, United States, 1/23/06. https://doi.org/10.1117/12.644939
Chao, Shih-Hui ; Holl, Mark R. ; McQuaide, Sarah C. ; Meldrum, Deirdre. / Oxygen concentration measurement with a phosphorescence lifetime based micro-sensor array using a digital light modulation microscope. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 6088 2006.
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