Overview of PCR

Christine D. Kuslich, Buena Chui, Carl T. Yamashiro

    Research output: Contribution to journalArticlepeer-review

    6 Scopus citations

    Abstract

    The polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique previously. In addition, a number of different applications utilizing the basic PCR technique will be described.

    Original languageEnglish (US)
    Article numbere27
    JournalCurrent Protocols in Essential Laboratory Techniques
    Volume18
    Issue number1
    DOIs
    StatePublished - Jun 2019

    Keywords

    • DNA amplification
    • PCR
    • PCR applications
    • PCR decontamination
    • PCR protocol
    • laboratory design
    • polymerase chain reaction
    • thermal cyclers

    ASJC Scopus subject areas

    • Chemistry (miscellaneous)
    • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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