Overview of PCR

Christine D. Kuslich; Buena Chui; Carl T. Yamashiro

doi:10.1002/cpet.27

Overview of PCR

Christine D. Kuslich, Buena Chui, Carl T. Yamashiro

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique previously. In addition, a number of different applications utilizing the basic PCR technique will be described.

Original language	English (US)
Article number	e27
Journal	Current Protocols in Essential Laboratory Techniques
Volume	18
Issue number	1
DOIs	https://doi.org/10.1002/cpet.27
State	Published - Jun 2019

Keywords

DNA amplification
PCR
PCR applications
PCR decontamination
PCR protocol
laboratory design
polymerase chain reaction
thermal cyclers

ASJC Scopus subject areas

Chemistry (miscellaneous)
Biochemistry, Genetics and Molecular Biology (miscellaneous)

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10.1002/cpet.27

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keywords = "DNA amplification, PCR, PCR applications, PCR decontamination, PCR protocol, laboratory design, polymerase chain reaction, thermal cyclers",

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AU - Chui, Buena

AU - Yamashiro, Carl T.

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AB - The polymerase chain reaction (PCR) has revolutionized molecular biology and has enabled many other technologies and applications commonly used in research, and is part of many life science products. This unit will cover the basic principles of PCR, including how to carry out PCR in a laboratory that has not used this powerful technique previously. In addition, a number of different applications utilizing the basic PCR technique will be described.

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KW - PCR

KW - PCR applications

KW - PCR decontamination

KW - PCR protocol

KW - laboratory design

KW - polymerase chain reaction

KW - thermal cyclers

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Overview of PCR

Abstract

Keywords

ASJC Scopus subject areas

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