Overview of Biochemical Assays in Lipidic Cubic Phase

Zhu Lan, Ming Yue Lee, Eugene Chun, Bin Liu, Wei Liu

Research output: Contribution to journalArticle

Abstract

The development of novel biochemical methods to efficiently characterize membrane protein (MP) properties in lipidic cubic phase (LCP) is important for studying complicated MPs and their multimeric complexes. Here, we summarize recent LCP-related assays and provide an outlook on their applications in structure and function studies of MPs.

Original languageEnglish (US)
JournalTrends in Biochemical Sciences
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Assays
Membrane Proteins

Keywords

  • crystallization
  • functional assay
  • LCP
  • membrane protein
  • protein stability

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Overview of Biochemical Assays in Lipidic Cubic Phase. / Lan, Zhu; Lee, Ming Yue; Chun, Eugene; Liu, Bin; Liu, Wei.

In: Trends in Biochemical Sciences, 01.01.2018.

Research output: Contribution to journalArticle

Lan, Zhu ; Lee, Ming Yue ; Chun, Eugene ; Liu, Bin ; Liu, Wei. / Overview of Biochemical Assays in Lipidic Cubic Phase. In: Trends in Biochemical Sciences. 2018.
@article{cfc5c33d2c9c44cd8885986a7cf44ed7,
title = "Overview of Biochemical Assays in Lipidic Cubic Phase",
abstract = "The development of novel biochemical methods to efficiently characterize membrane protein (MP) properties in lipidic cubic phase (LCP) is important for studying complicated MPs and their multimeric complexes. Here, we summarize recent LCP-related assays and provide an outlook on their applications in structure and function studies of MPs.",
keywords = "crystallization, functional assay, LCP, membrane protein, protein stability",
author = "Zhu Lan and Lee, {Ming Yue} and Eugene Chun and Bin Liu and Wei Liu",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.tibs.2018.08.008",
language = "English (US)",
journal = "Trends in Biochemical Sciences",
issn = "0376-5067",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Overview of Biochemical Assays in Lipidic Cubic Phase

AU - Lan, Zhu

AU - Lee, Ming Yue

AU - Chun, Eugene

AU - Liu, Bin

AU - Liu, Wei

PY - 2018/1/1

Y1 - 2018/1/1

N2 - The development of novel biochemical methods to efficiently characterize membrane protein (MP) properties in lipidic cubic phase (LCP) is important for studying complicated MPs and their multimeric complexes. Here, we summarize recent LCP-related assays and provide an outlook on their applications in structure and function studies of MPs.

AB - The development of novel biochemical methods to efficiently characterize membrane protein (MP) properties in lipidic cubic phase (LCP) is important for studying complicated MPs and their multimeric complexes. Here, we summarize recent LCP-related assays and provide an outlook on their applications in structure and function studies of MPs.

KW - crystallization

KW - functional assay

KW - LCP

KW - membrane protein

KW - protein stability

UR - http://www.scopus.com/inward/record.url?scp=85053657482&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053657482&partnerID=8YFLogxK

U2 - 10.1016/j.tibs.2018.08.008

DO - 10.1016/j.tibs.2018.08.008

M3 - Article

C2 - 30243833

AN - SCOPUS:85053657482

JO - Trends in Biochemical Sciences

JF - Trends in Biochemical Sciences

SN - 0376-5067

ER -