Overexpression of BDNF in the ventral tegmental area enhances binge cocaine self-administration in rats exposed to repeated social defeat

Junshi Wang, Ryan M. Bastle, Caroline E. Bass, Ronald P. Hammer, Janet Neisewander, Ella M. Nikulina

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Stress is a major risk factor for substance abuse. Intermittent social defeat stress increases drug self-administration (SA) and elevates brain-derived neurotrophic factor (BDNF) expression in the ventral tegmental area (VTA) in rats. Intra-VTA BDNF overexpression enhances social defeat stress-induced cross-sensitization to psychostimulants and induces nucleus accumbens (NAc) ΔFosB expression. Therefore, increased VTA BDNF may mimic or augment the development of drug abuse-related behavior following social stress. To test this hypothesis, adeno-associated virus (AAV) was infused into the VTA to overexpress either GFP alone (control) or GFP + BDNF. Rats were then either handled or exposed to intermittent social defeat stress before beginning cocaine SA training. The SA acquisition and maintenance phases were followed by testing on a progressive ratio (PR) schedule of cocaine reinforcement, and then during a 12-h access “binge” cocaine SA session. BDNF and ΔFosB were quantified postmortem in regions of the mesocorticolimbic circuitry using immunohistochemistry. Social defeat stress increased cocaine intake on a PR schedule, regardless of virus treatment. While stress alone increased intake during the 12-h binge session, socially-defeated rats that received VTA BDNF overexpression exhibited even greater cocaine intake compared to the GFP-stressed group. However, VTA BDNF overexpression alone did not alter binge intake. BDNF expression in the VTA was also positively correlated with total cocaine intake during binge session. VTA BDNF overexpression increased ΔFosB expression in the NAc, but not in the dorsal striatum. Here we demonstrate that VTA BDNF overexpression increases long-access cocaine intake, but only under stressful conditions. Therefore, enhanced VTA-BDNF expression may be a facilitator for stress-induced increases in drug abuse-related behavior specifically under conditions that capture compulsive-like drug intake.

Original languageEnglish (US)
Pages (from-to)121-130
Number of pages10
JournalNeuropharmacology
Volume109
DOIs
StatePublished - Oct 1 2016

Fingerprint

Ventral Tegmental Area
Self Administration
Brain-Derived Neurotrophic Factor
Cocaine
Substance-Related Disorders
Nucleus Accumbens
Reinforcement Schedule
Dependovirus
Pharmaceutical Preparations
Appointments and Schedules
Immunohistochemistry
Maintenance
Viruses

Keywords

  • Brain-derived neurotrophic factor
  • Cocaine self-administration
  • deltaFosB
  • Nucleus accumbens
  • Social defeat stress

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Overexpression of BDNF in the ventral tegmental area enhances binge cocaine self-administration in rats exposed to repeated social defeat. / Wang, Junshi; Bastle, Ryan M.; Bass, Caroline E.; Hammer, Ronald P.; Neisewander, Janet; Nikulina, Ella M.

In: Neuropharmacology, Vol. 109, 01.10.2016, p. 121-130.

Research output: Contribution to journalArticle

Wang, Junshi ; Bastle, Ryan M. ; Bass, Caroline E. ; Hammer, Ronald P. ; Neisewander, Janet ; Nikulina, Ella M. / Overexpression of BDNF in the ventral tegmental area enhances binge cocaine self-administration in rats exposed to repeated social defeat. In: Neuropharmacology. 2016 ; Vol. 109. pp. 121-130.
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