Orientation of pigments in the thylakoid membrane and in the isolated chlorophyll-protein complexes of higher plants. I. Determination of optimal conditions for linear dichroism measurement

Philip Haworth, Charles J. Arntzen, Pierre Tapie, Jacques Breton

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The nature and possible causes of polarized light-scattering artefacts in linear dichroism measurements are investigated. Using criteria described in this article, the available orientation techniques have been critically assessed in order to obtain the linear dichroism spectra of thylakoids and of pigment-protein complexes isolated from pea. It is demonstrated here that the polyacrylamide gel squeezing technique of Abdourakhmanov et al. (Abdourakhmanov, I.A., Ganago, A.O., Erokhim, Yu.E., Solov'ev, A.A. and Chugunov, V.A. (1979) Biochim. Biophys. Acta 546, 183-186) does not lead to pigment degradation and that the linear dichroism spectra obtained in these conditions are essentially free of scattering artefacts. The linear dichroism spectra of light-harvesting complex isolated in different states of aggregation or incorporated into phospholipid vesicles are compared to the spectra of thylakoids. This comparison indicates: (1) that the isolation procedure of Burke et al. (Burke, J.J., Ditto, C.L. and Arntzen, C.J. (1978) Arch. Biochem. Biophys. 187, 252-263) leads to light-harvesting complex in which the in vivo orientation of pigments is preserved; (2) that the antenna chlorophyll a molecules of this complex have a significant degree of orientation with respect to the plane of the thylakoid.

Original languageEnglish (US)
Pages (from-to)428-435
Number of pages8
JournalBBA - Bioenergetics
Volume679
Issue number3
DOIs
StatePublished - Mar 16 1982

Keywords

  • (Pea chloroplast)
  • Chlorophyl-protein complex
  • Linear dichroism
  • Pigment orientation
  • Polarized light
  • Thylakoid membrane

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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