TY - JOUR
T1 - Orange Carotenoid Protein as a Control Element in an Antenna System Based on a DNA Nanostructure
AU - Andreoni, Alessio
AU - Lin, Su
AU - Liu, Haijun
AU - Blankenship, Robert E.
AU - Yan, Hao
AU - Woodbury, Neal
N1 - Funding Information:
This work was funded by the DOD MURI award W911NF-12-1-0420 (A.A. S.L., H.Y., and N.W.W.) and NSF grants MCB-1157788 (A.A. and S.L.) and the Department of Energy Office of Basic Energy Sciences (grant no. DE-FG02-07ER15902; H.L. and R.E.B.).
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/2/8
Y1 - 2017/2/8
N2 - Taking inspiration from photosynthetic mechanisms in natural systems, we introduced a light-sensitive photo protective quenching element to an artificial light-harvesting antenna model to control the flow of energy as a function of light intensity excitation. The orange carotenoid protein (OCP) is a nonphotochemical quencher in cyanobacteria: under high-light conditions, the protein undergoes a spectral shift, and by binding to the phycobilisome, it absorbs excess light and dissipates it as heat. By the use of DNA as a scaffold, an antenna system made of organic dyes (Cy3 and Cy5) was constructed, and OCP was assembled on it as a modulated quenching element. By controlling the illumination intensity, it is possible to switch the direction of excitation energy transfer from the donor Cy3 to either of two acceptors. Under low-light conditions, energy is transferred from Cy3 to Cy5, and under intense illumination, energy is partially transferred to OCP as well. These results demonstrate the feasibility of controlling the pathway of energy transfer using light intensity in an engineered light-harvesting system.
AB - Taking inspiration from photosynthetic mechanisms in natural systems, we introduced a light-sensitive photo protective quenching element to an artificial light-harvesting antenna model to control the flow of energy as a function of light intensity excitation. The orange carotenoid protein (OCP) is a nonphotochemical quencher in cyanobacteria: under high-light conditions, the protein undergoes a spectral shift, and by binding to the phycobilisome, it absorbs excess light and dissipates it as heat. By the use of DNA as a scaffold, an antenna system made of organic dyes (Cy3 and Cy5) was constructed, and OCP was assembled on it as a modulated quenching element. By controlling the illumination intensity, it is possible to switch the direction of excitation energy transfer from the donor Cy3 to either of two acceptors. Under low-light conditions, energy is transferred from Cy3 to Cy5, and under intense illumination, energy is partially transferred to OCP as well. These results demonstrate the feasibility of controlling the pathway of energy transfer using light intensity in an engineered light-harvesting system.
KW - DNA nanotechnology
KW - Orange carotenoid protein
KW - artificial light-harvesting
KW - energy transfer
KW - fluorescence spectroscopy
KW - photoprotection
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U2 - 10.1021/acs.nanolett.6b04846
DO - 10.1021/acs.nanolett.6b04846
M3 - Article
C2 - 28081606
AN - SCOPUS:85011999826
SN - 1530-6984
VL - 17
SP - 1174
EP - 1180
JO - Nano Letters
JF - Nano Letters
IS - 2
ER -