Oral vaccination with different antigens from Yersinia pestis KIM delivered by live attenuated Salmonella typhimurium elicits a protective immune response against plague

Christine G. Branger, Roy Curtiss, Robert D. Perry, Jacqueline D. Fetherston

Research output: Chapter in Book/Report/Conference proceedingConference contribution

26 Scopus citations

Abstract

The use of live recombinant Salmonella attenuated vaccine (RASV) encoding Yersinia proteins is a promising new approach for the vaccination against Yersinia pestis. We have tested the efficacy of 2 proteins, Psn and a portion of LcrV in protecting mice against virulent Yersinia pestis challenge. To remove the immunosuppressive properties of LcrV protein, the lcrV gene, without the TLR2 receptor sequence, was cloned into a β-lactamase secretion vector. Immunizations were performed with RSAV expressing LcrV or Psn. Challenge with a virulent Y. pestis strain was performed 4 weeks after the last immunization. Our results show that the truncated LcrV protein delivered by RASV is sufficient to afford a full protective immune response in a mouse model of bubonic plague and the Psn protein afforded partial protection in a non-optimized system. This finding should facilitate the design and development of a new generation of vaccines against Y. pestis.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer New York
Pages387-399
Number of pages13
Volume603
ISBN (Print)9780387721231
DOIs
StatePublished - 2007

Publication series

NameAdvances in Experimental Medicine and Biology
Volume603
ISSN (Print)00652598

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Branger, C. G., Curtiss, R., Perry, R. D., & Fetherston, J. D. (2007). Oral vaccination with different antigens from Yersinia pestis KIM delivered by live attenuated Salmonella typhimurium elicits a protective immune response against plague. In Advances in Experimental Medicine and Biology (Vol. 603, pp. 387-399). (Advances in Experimental Medicine and Biology; Vol. 603). Springer New York. https://doi.org/10.1007/978-0-387-72124-8_36