Oral immunization with attenuated Salmonella expressing human sperm antigen induces antibodies in serum and the reproductive tract

J. Srinivasan, S. Tinge, R. Wright, J. C. Herr, R. Curtiss

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Induction of immune responses in the reproductive tract will be crucial for a functional gamete antigen-based antifertility vaccine. Here we describe the construction and development of an avirulent Salmonella as an oral vaccine delivery vector to elicit sperm-specific immune responses in reproductive tract secretions. A cDNA sequence encoding the human sperm antigen SP10 was cloned on an asd + vector and expressed to a high level in an avirulent Δcya, Δcrp, and Δasd vaccine strain of Salmonella typhimurium. Oral immunization of female BALB/c mice with this recombinant Salmonella elicited high-titer anti-SP10 IgG antibodies in serum and IgA antibodies in vaginal secretions. Anti-SP10 antibody titers could be increased by secondary and tertiary oral administrations of the recombinant Salmonella. Induction of sperm-specific antibodies in the reproductive tract following oral administration of a recombinant Salmonella could lead to the development of a simple, safe, efficient, and easy-to-use antifertility vaccine.

Original languageEnglish (US)
Pages (from-to)462-471
Number of pages10
JournalBiology of Reproduction
Volume53
Issue number2
DOIs
StatePublished - Aug 30 1995

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ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

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