TY - JOUR
T1 - Oral immunization with attenuated Salmonella expressing human sperm antigen induces antibodies in serum and the reproductive tract
AU - Srinivasan, J.
AU - Tinge, S.
AU - Wright, R.
AU - Herr, J. C.
AU - Curtiss, R.
PY - 1995
Y1 - 1995
N2 - Induction of immune responses in the reproductive tract will be crucial for a functional gamete antigen-based antifertility vaccine. Here we describe the construction and development of an avirulent Salmonella as an oral vaccine delivery vector to elicit sperm-specific immune responses in reproductive tract secretions. A cDNA sequence encoding the human sperm antigen SP10 was cloned on an asd + vector and expressed to a high level in an avirulent Δcya, Δcrp, and Δasd vaccine strain of Salmonella typhimurium. Oral immunization of female BALB/c mice with this recombinant Salmonella elicited high-titer anti-SP10 IgG antibodies in serum and IgA antibodies in vaginal secretions. Anti-SP10 antibody titers could be increased by secondary and tertiary oral administrations of the recombinant Salmonella. Induction of sperm-specific antibodies in the reproductive tract following oral administration of a recombinant Salmonella could lead to the development of a simple, safe, efficient, and easy-to-use antifertility vaccine.
AB - Induction of immune responses in the reproductive tract will be crucial for a functional gamete antigen-based antifertility vaccine. Here we describe the construction and development of an avirulent Salmonella as an oral vaccine delivery vector to elicit sperm-specific immune responses in reproductive tract secretions. A cDNA sequence encoding the human sperm antigen SP10 was cloned on an asd + vector and expressed to a high level in an avirulent Δcya, Δcrp, and Δasd vaccine strain of Salmonella typhimurium. Oral immunization of female BALB/c mice with this recombinant Salmonella elicited high-titer anti-SP10 IgG antibodies in serum and IgA antibodies in vaginal secretions. Anti-SP10 antibody titers could be increased by secondary and tertiary oral administrations of the recombinant Salmonella. Induction of sperm-specific antibodies in the reproductive tract following oral administration of a recombinant Salmonella could lead to the development of a simple, safe, efficient, and easy-to-use antifertility vaccine.
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U2 - 10.1095/biolreprod53.2.462
DO - 10.1095/biolreprod53.2.462
M3 - Article
C2 - 7492701
AN - SCOPUS:0029119680
SN - 0006-3363
VL - 53
SP - 462
EP - 471
JO - Biology of Reproduction
JF - Biology of Reproduction
IS - 2
ER -