Oral immunization with a recombinant bacterial antigen produced in transgenic plants

Tariq A. Haq, Hugh Mason, John D. Clements, Charles J. Arntzen

Research output: Contribution to journalArticle

503 Citations (Scopus)

Abstract

The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.

Original languageEnglish (US)
Pages (from-to)714-716
Number of pages3
JournalScience
Volume268
Issue number5211
StatePublished - 1995
Externally publishedYes

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Bacterial Antigens
Enterotoxins
Genetically Modified Plants
Solanum tuberosum
Immunization
Cytoprotection
Transgenic Mice
Tobacco
Hot Temperature
Escherichia coli
Ligands
Peptides
Serum
Genes
IgA receptor
immunoglobulin B

ASJC Scopus subject areas

  • General

Cite this

Haq, T. A., Mason, H., Clements, J. D., & Arntzen, C. J. (1995). Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science, 268(5211), 714-716.

Oral immunization with a recombinant bacterial antigen produced in transgenic plants. / Haq, Tariq A.; Mason, Hugh; Clements, John D.; Arntzen, Charles J.

In: Science, Vol. 268, No. 5211, 1995, p. 714-716.

Research output: Contribution to journalArticle

Haq, TA, Mason, H, Clements, JD & Arntzen, CJ 1995, 'Oral immunization with a recombinant bacterial antigen produced in transgenic plants', Science, vol. 268, no. 5211, pp. 714-716.
Haq, Tariq A. ; Mason, Hugh ; Clements, John D. ; Arntzen, Charles J. / Oral immunization with a recombinant bacterial antigen produced in transgenic plants. In: Science. 1995 ; Vol. 268, No. 5211. pp. 714-716.
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