Oral immunization with a recombinant bacterial antigen produced in transgenic plants

Tariq A. Haq, Hugh S. Mason, John D. Clements, Charles J. Arntzen

Research output: Contribution to journalArticle

511 Scopus citations

Abstract

The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.

Original languageEnglish (US)
Pages (from-to)714-716
Number of pages3
JournalScience
Volume268
Issue number5211
DOIs
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Oral immunization with a recombinant bacterial antigen produced in transgenic plants'. Together they form a unique fingerprint.

  • Cite this