Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague

Wei Sun, Shilpa Sanapala, Hannah Rahav, Roy Curtiss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A Yersinia pseudotuberculosis PB1+ (Yptb PB1+) mutant strain combined with chromosome insertion of the caf1R-caf1A-caf1M-caf1 operon and deletions of yopJ and yopK, χ10068 [pYV-ω2 (δyopJ315 δyopK108) δlacZ044::caf1R-caf1M-caf1A-caf1] was constructed. Results indicated that gene insertion and deletion did not affect the growth rate of χ10068 compared to wild-type Yptb cultured at 26°C. In addition, the F1 antigen in χ10068 was synthesized and secreted on the surface of bacteria at 37°C (mammalian body temperature), not at ambient culture temperature (26°C). Immunization with χ10068 primed antibody responses and specific T-cell responses to F1 and YpL (Y. pestis whole cell lysate). Oral immunization with a single dose of χ10068 provided 70% protection against a subcutaneous (s.c.) challenge with ~2.6×105 LD50 of Y. pestis KIM6+ (pCD1Ap) (KIM6+Ap) and 90% protection against an intranasal (i.n.) challenge with ~500 LD50 of KIM6+Ap in mice. Our results suggest that χ10068 can be used as an effective precursor to make a safe vaccine to prevent plague in humans and to eliminate plague circulation among humans and animals.

Original languageEnglish (US)
Pages (from-to)6727-6735
Number of pages9
JournalVaccine
Volume33
Issue number48
DOIs
StatePublished - Nov 27 2015

Fingerprint

Yersinia pseudotuberculosis
Plague
plague
Lethal Dose 50
lethal dose 50
oral administration
Oral Administration
Immunity
immunity
Immunization
oral vaccination
Insertional Mutagenesis
Gene Deletion
operon
Operon
Body Temperature
body temperature
Antibody Formation
immunization
Vaccines

Keywords

  • F1
  • Plague
  • Vaccine
  • Yersinia pseudotuberculosis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague. / Sun, Wei; Sanapala, Shilpa; Rahav, Hannah; Curtiss, Roy.

In: Vaccine, Vol. 33, No. 48, 27.11.2015, p. 6727-6735.

Research output: Contribution to journalArticle

Sun, Wei ; Sanapala, Shilpa ; Rahav, Hannah ; Curtiss, Roy. / Oral administration of a recombinant attenuated Yersinia pseudotuberculosis strain elicits protective immunity against plague. In: Vaccine. 2015 ; Vol. 33, No. 48. pp. 6727-6735.
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