Optimal hepatitis B virus primer sequence design for isothermal amplification

Szu Yuan Lee, Chun Nan Lee, Holl Mark, Deirdre Meldrum, Chih Kung Lee, Chii Wann Lin

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have reported the successful use of the Loop Mediated Isothermal Amplification (LAMP) reaction for Hepatitis B Virus (HBV) DNA in a 25 μl micro-reactor for the development of a rapid diagnostic device recently. This method shows the successful amplification of HBV DNA with high specificity and sensitivity efficiently within one hour. Such a powerful method can be used for many other fast diagnostic applications with the help of optimal primer design and miniaturization of reactor. In this report, we will first summarize the general design rules for primer design from literature reviews and our experimental results. We will discuss the effects of HBV primers and concentrations of working buffers for optimal performance.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalBiomedical Engineering - Applications, Basis and Communications
Volume19
Issue number3
DOIs
StatePublished - Jun 2007

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Viruses
Hepatitis B virus
Amplification
DNA
Miniaturization
Buffers
Sensitivity and Specificity
Equipment and Supplies

Keywords

  • Hepatitis B virus
  • Isothermal
  • LAMP
  • Primer design

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Optimal hepatitis B virus primer sequence design for isothermal amplification. / Lee, Szu Yuan; Lee, Chun Nan; Mark, Holl; Meldrum, Deirdre; Lee, Chih Kung; Lin, Chii Wann.

In: Biomedical Engineering - Applications, Basis and Communications, Vol. 19, No. 3, 06.2007, p. 137-144.

Research output: Contribution to journalArticle

Lee, Szu Yuan ; Lee, Chun Nan ; Mark, Holl ; Meldrum, Deirdre ; Lee, Chih Kung ; Lin, Chii Wann. / Optimal hepatitis B virus primer sequence design for isothermal amplification. In: Biomedical Engineering - Applications, Basis and Communications. 2007 ; Vol. 19, No. 3. pp. 137-144.
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