Optimal hepatitis B virus primer sequence design for isothermal amplification

Szu Yuan Lee, Chun Nan Lee, Holl Mark, Deirdre Meldrum, Chih Kung Lee, Chii Wann Lin

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have reported the successful use of the Loop Mediated Isothermal Amplification (LAMP) reaction for Hepatitis B Virus (HBV) DNA in a 25 μl micro-reactor for the development of a rapid diagnostic device recently. This method shows the successful amplification of HBV DNA with high specificity and sensitivity efficiently within one hour. Such a powerful method can be used for many other fast diagnostic applications with the help of optimal primer design and miniaturization of reactor. In this report, we will first summarize the general design rules for primer design from literature reviews and our experimental results. We will discuss the effects of HBV primers and concentrations of working buffers for optimal performance.

Original languageEnglish (US)
Pages (from-to)137-144
Number of pages8
JournalBiomedical Engineering - Applications, Basis and Communications
Volume19
Issue number3
DOIs
StatePublished - Jun 2007

Keywords

  • Hepatitis B virus
  • Isothermal
  • LAMP
  • Primer design

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomedical Engineering

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