Abstract
Optically patterned cell growth was applied to the direct evolution of a green fluorescent protein (GFP) based glucose sensor. The fluorescent property of green proteins was utilized for development of the biosensor. The gene was modified so that it would bind a glucose molecule and couple its fluorescence properties to the binding event. A methodology called patchwork PCR, utilizing a combination of the PCR and DNA shuffling techniques was used for mutagenesis.
| Original language | English (US) |
|---|---|
| Title of host publication | Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS |
| Pages | 322-323 |
| Number of pages | 2 |
| Volume | 1 |
| State | Published - 2001 |
| Event | 14th Annual Meeting of the IEEE Lasers and Electro-Optics Society - San Diego, CA, United States Duration: Nov 11 2001 → Nov 15 2001 |
Other
| Other | 14th Annual Meeting of the IEEE Lasers and Electro-Optics Society |
|---|---|
| Country/Territory | United States |
| City | San Diego, CA |
| Period | 11/11/01 → 11/15/01 |
ASJC Scopus subject areas
- Control and Systems Engineering
- Electrical and Electronic Engineering
- Industrial and Manufacturing Engineering