Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor

A. Scruggs, Jennie Bever, B. Bowen, N. Woodbury

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optically patterned cell growth was applied to the direct evolution of a green fluorescent protein (GFP) based glucose sensor. The fluorescent property of green proteins was utilized for development of the biosensor. The gene was modified so that it would bind a glucose molecule and couple its fluorescence properties to the binding event. A methodology called patchwork PCR, utilizing a combination of the PCR and DNA shuffling techniques was used for mutagenesis.

Original languageEnglish (US)
Title of host publicationConference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS
Pages322-323
Number of pages2
Volume1
StatePublished - 2001
Event14th Annual Meeting of the IEEE Lasers and Electro-Optics Society - San Diego, CA, United States
Duration: Nov 11 2001Nov 15 2001

Other

Other14th Annual Meeting of the IEEE Lasers and Electro-Optics Society
CountryUnited States
CitySan Diego, CA
Period11/11/0111/15/01

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Industrial and Manufacturing Engineering

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  • Cite this

    Scruggs, A., Bever, J., Bowen, B., & Woodbury, N. (2001). Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. In Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS (Vol. 1, pp. 322-323)