Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor

A. Scruggs, Jennie Bever, B. Bowen, N. Woodbury

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optically patterned cell growth was applied to the direct evolution of a green fluorescent protein (GFP) based glucose sensor. The fluorescent property of green proteins was utilized for development of the biosensor. The gene was modified so that it would bind a glucose molecule and couple its fluorescence properties to the binding event. A methodology called patchwork PCR, utilizing a combination of the PCR and DNA shuffling techniques was used for mutagenesis.

Original languageEnglish (US)
Title of host publicationConference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS
Pages322-323
Number of pages2
Volume1
StatePublished - 2001
Event14th Annual Meeting of the IEEE Lasers and Electro-Optics Society - San Diego, CA, United States
Duration: Nov 11 2001Nov 15 2001

Other

Other14th Annual Meeting of the IEEE Lasers and Electro-Optics Society
CountryUnited States
CitySan Diego, CA
Period11/11/0111/15/01

Fingerprint

Glucose sensors
Cell growth
Proteins
Mutagenesis
Biosensors
Glucose
DNA
Genes
Fluorescence
Molecules

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Industrial and Manufacturing Engineering

Cite this

Scruggs, A., Bever, J., Bowen, B., & Woodbury, N. (2001). Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. In Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS (Vol. 1, pp. 322-323)

Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. / Scruggs, A.; Bever, Jennie; Bowen, B.; Woodbury, N.

Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS. Vol. 1 2001. p. 322-323.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Scruggs, A, Bever, J, Bowen, B & Woodbury, N 2001, Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. in Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS. vol. 1, pp. 322-323, 14th Annual Meeting of the IEEE Lasers and Electro-Optics Society, San Diego, CA, United States, 11/11/01.
Scruggs A, Bever J, Bowen B, Woodbury N. Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. In Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS. Vol. 1. 2001. p. 322-323
Scruggs, A. ; Bever, Jennie ; Bowen, B. ; Woodbury, N. / Optically patterned cell growth applied to the directed evolution of a GFP-based glucose sensor. Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS. Vol. 1 2001. pp. 322-323
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