One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA

Sm Z. Khaled, Armando Cevenini, Iman K. Yazdi, Alessandro Parodi, Michael Evangelopoulos, Claudia Corbo, Shilpa Scaria, Ye Hu, Seth G. Haddix, Bruna Corradetti, Francesco Salvatore, Ennio Tasciotti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This report describes a novel, one-pot synthesis of hybrid nanoparticles formed by a nanostructured inorganic silica core and an organic pH-responsive hydrogel shell. This easy-to-perform, oil-in-water emulsion process synthesizes fluorescently-doped silica nanoparticles wrapped within a tunable coating of cationic poly(2-diethylaminoethyl methacrylate) hydrogel in one step. Transmission electron microscopy and dynamic light scattering analysis demonstrated that the hydrogel-coated nanoparticles are uniformly dispersed in the aqueous phase. The formation of covalent chemical bonds between the silica and the polymer increases the stability of the organic phase around the inorganic core as demonstrated by thermogravimetric analysis. The cationic nature of the hydrogel is responsible for the pH buffering properties of the nanostructured system and was evaluated by titration experiments. Zeta-potential analysis demonstrated that the charge of the system was reversed when transitioned from acidic to basic pH and vice versa. Consequently, small interfering RNA (siRNA) can be loaded and released in an acidic pH environment thereby enabling the hybrid particles and their payload to avoid endosomal sequestration and enzymatic degradation. These nanoparticles, loaded with specific siRNA molecules directed towards the transcript of the membrane receptor CXCR4, significantly decreased the expression of this protein in a human breast cancer cell line (i.e., MDA-MB-231). Moreover, intravenous administration of siRNA-loaded nanoparticles demonstrated a preferential accumulation at the tumor site that resulted in a reduction of CXCR4 expression.

Original languageEnglish (US)
Pages (from-to)57-68
Number of pages12
JournalBiomaterials
Volume87
DOIs
StatePublished - May 1 2016
Externally publishedYes

Fingerprint

RNA
Nanoparticles
Small Interfering RNA
Hydrogel
Hydrogels
Silicon Dioxide
Silica
CXCR4 Receptors
Chemical bonds
Dynamic light scattering
Zeta potential
Emulsions
Transmission Electron Microscopy
Titration
Intravenous Administration
Thermogravimetric analysis
Tumors
Polymers
Oils
Cells

Keywords

  • Cancer therapy
  • Lysosomal escape
  • Nanoparticles
  • PH-responsive polymer
  • SiRNA delivery

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Khaled, S. Z., Cevenini, A., Yazdi, I. K., Parodi, A., Evangelopoulos, M., Corbo, C., ... Tasciotti, E. (2016). One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA. Biomaterials, 87, 57-68. https://doi.org/10.1016/j.biomaterials.2016.01.052

One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA. / Khaled, Sm Z.; Cevenini, Armando; Yazdi, Iman K.; Parodi, Alessandro; Evangelopoulos, Michael; Corbo, Claudia; Scaria, Shilpa; Hu, Ye; Haddix, Seth G.; Corradetti, Bruna; Salvatore, Francesco; Tasciotti, Ennio.

In: Biomaterials, Vol. 87, 01.05.2016, p. 57-68.

Research output: Contribution to journalArticle

Khaled, SZ, Cevenini, A, Yazdi, IK, Parodi, A, Evangelopoulos, M, Corbo, C, Scaria, S, Hu, Y, Haddix, SG, Corradetti, B, Salvatore, F & Tasciotti, E 2016, 'One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA', Biomaterials, vol. 87, pp. 57-68. https://doi.org/10.1016/j.biomaterials.2016.01.052
Khaled, Sm Z. ; Cevenini, Armando ; Yazdi, Iman K. ; Parodi, Alessandro ; Evangelopoulos, Michael ; Corbo, Claudia ; Scaria, Shilpa ; Hu, Ye ; Haddix, Seth G. ; Corradetti, Bruna ; Salvatore, Francesco ; Tasciotti, Ennio. / One-pot synthesis of pH-responsive hybrid nanogel particles for the intracellular delivery of small interfering RNA. In: Biomaterials. 2016 ; Vol. 87. pp. 57-68.
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