Okra enation leaf curl virus in Papaya from Iran displaying severe leaf curl symptoms

K. Bananej, S. Kraberger, Arvind Varsani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During a survey of the Bahoo-Kalat and Zar-Abad areas of Iran in February 2010,papaya plants were found with severe leaf curling and vein swelling. A begomovirus (family Geminiviridae) was suspected of being responsible for the disease,so begomovirus-specific degenerate primers and PCR on total DNA were used to obtain amplicons. Based on the sequence of these amplicons,abutting primers were designed to recover the complete begomovirus from seven PCR positive papaya plants. Seven distinct begomovirus molecules (2746 nt each) were recovered that share 85.5-91.6% genome-wide sequence identity with Okra enation leaf curl virus (OELCuV) isolates. Phylogenetic analyses with available OELCuV sequences support the identification of the papaya-infecting begomovirus from Iran as an isolate of OELCuV. To our knowledge this is the first report of a begomovirus infecting papaya in Iran and the first record of papaya as host of OELCuV.

Original languageEnglish (US)
JournalJournal of Plant Pathology
Volume98
Issue number3
DOIs
StatePublished - 2016
Externally publishedYes

Fingerprint

Papaya leaf curl virus
Begomovirus
leaf curling
okra
signs and symptoms (plants)
papayas
Iran
viruses
Geminiviridae
plant veins
genome

Keywords

  • Begomovirus
  • Leaf curling
  • Papaya

ASJC Scopus subject areas

  • Plant Science

Cite this

Okra enation leaf curl virus in Papaya from Iran displaying severe leaf curl symptoms. / Bananej, K.; Kraberger, S.; Varsani, Arvind.

In: Journal of Plant Pathology, Vol. 98, No. 3, 2016.

Research output: Contribution to journalArticle

@article{2c62dea5dcc84906aa1380cc64d2c3bf,
title = "Okra enation leaf curl virus in Papaya from Iran displaying severe leaf curl symptoms",
abstract = "During a survey of the Bahoo-Kalat and Zar-Abad areas of Iran in February 2010,papaya plants were found with severe leaf curling and vein swelling. A begomovirus (family Geminiviridae) was suspected of being responsible for the disease,so begomovirus-specific degenerate primers and PCR on total DNA were used to obtain amplicons. Based on the sequence of these amplicons,abutting primers were designed to recover the complete begomovirus from seven PCR positive papaya plants. Seven distinct begomovirus molecules (2746 nt each) were recovered that share 85.5-91.6{\%} genome-wide sequence identity with Okra enation leaf curl virus (OELCuV) isolates. Phylogenetic analyses with available OELCuV sequences support the identification of the papaya-infecting begomovirus from Iran as an isolate of OELCuV. To our knowledge this is the first report of a begomovirus infecting papaya in Iran and the first record of papaya as host of OELCuV.",
keywords = "Begomovirus, Leaf curling, Papaya",
author = "K. Bananej and S. Kraberger and Arvind Varsani",
year = "2016",
doi = "10.4454/JPP.V98I3.043",
language = "English (US)",
volume = "98",
journal = "Journal of Plant Pathology",
issn = "1125-4653",
publisher = "Edizioni E.T.S.",
number = "3",

}

TY - JOUR

T1 - Okra enation leaf curl virus in Papaya from Iran displaying severe leaf curl symptoms

AU - Bananej, K.

AU - Kraberger, S.

AU - Varsani, Arvind

PY - 2016

Y1 - 2016

N2 - During a survey of the Bahoo-Kalat and Zar-Abad areas of Iran in February 2010,papaya plants were found with severe leaf curling and vein swelling. A begomovirus (family Geminiviridae) was suspected of being responsible for the disease,so begomovirus-specific degenerate primers and PCR on total DNA were used to obtain amplicons. Based on the sequence of these amplicons,abutting primers were designed to recover the complete begomovirus from seven PCR positive papaya plants. Seven distinct begomovirus molecules (2746 nt each) were recovered that share 85.5-91.6% genome-wide sequence identity with Okra enation leaf curl virus (OELCuV) isolates. Phylogenetic analyses with available OELCuV sequences support the identification of the papaya-infecting begomovirus from Iran as an isolate of OELCuV. To our knowledge this is the first report of a begomovirus infecting papaya in Iran and the first record of papaya as host of OELCuV.

AB - During a survey of the Bahoo-Kalat and Zar-Abad areas of Iran in February 2010,papaya plants were found with severe leaf curling and vein swelling. A begomovirus (family Geminiviridae) was suspected of being responsible for the disease,so begomovirus-specific degenerate primers and PCR on total DNA were used to obtain amplicons. Based on the sequence of these amplicons,abutting primers were designed to recover the complete begomovirus from seven PCR positive papaya plants. Seven distinct begomovirus molecules (2746 nt each) were recovered that share 85.5-91.6% genome-wide sequence identity with Okra enation leaf curl virus (OELCuV) isolates. Phylogenetic analyses with available OELCuV sequences support the identification of the papaya-infecting begomovirus from Iran as an isolate of OELCuV. To our knowledge this is the first report of a begomovirus infecting papaya in Iran and the first record of papaya as host of OELCuV.

KW - Begomovirus

KW - Leaf curling

KW - Papaya

UR - http://www.scopus.com/inward/record.url?scp=84992386927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84992386927&partnerID=8YFLogxK

U2 - 10.4454/JPP.V98I3.043

DO - 10.4454/JPP.V98I3.043

M3 - Article

AN - SCOPUS:84992386927

VL - 98

JO - Journal of Plant Pathology

JF - Journal of Plant Pathology

SN - 1125-4653

IS - 3

ER -