@article{5350316e88a24f00ad64d7ab139ac931,
title = "Off-target capture data, endosymbiont genes and morphology reveal a relict lineage that is sister to all other singing cicadas",
abstract = "Phylogenetic asymmetry is common throughout the tree of life and results from contrasting patterns of speciation and extinction in the paired descendant lineages of ancestral nodes. On the depauperate side of a node, we find extant 'relict' taxa that sit atop long, unbranched lineages. Here, we show that a tiny, pale green, inconspicuous and poorly known cicada in the genus Derotettix, endemic to degraded salt-plain habitats in arid regions of central Argentina, is a relict lineage that is sister to all other modern cicadas. Nuclear and mitochondrial phylogenies of cicadas inferred from probe-based genomic hybrid capture data of both target and non-target loci and a morphological cladogram support this hypothesis. We strengthen this conclusion with genomic data from one of the cicada nutritional bacterial endosymbionts, Sulcia, an ancient and obligate endosymbiont of the larger plant-sucking bugs (Auchenorrhyncha) and an important source of maternally inherited phylogenetic data. We establish Derotettiginae subfam. nov. as a new, monogeneric, fifth cicada subfamily, and compile existing and new data on the distribution, ecology and diet of Derotettix. Our consideration of the palaeoenvironmental literature and host-plant phylogenetics allows us to predict what might have led to the relict status of Derotettix over 100 Myr of habitat change in South America.",
keywords = "Amaranthaceae, Argentina, Derotettiginae, Derotettix, South America, Sulcia, anchored hybrid enrichment, hybrid capture bycatch, palaeobiology, phylogenomics",
author = "Chris Simon and Gordon, {Eric R.L.} and Moulds, {M. S.} and Cole, {Jeffrey A.} and Diler Haji and Lemmon, {Alan R.} and Lemmon, {Emily Moriarty} and Michelle Kortyna and Katherine Nazario and Wade, {Elizabeth J.} and Meister, {Russell C.} and Geert Goemans and Chiswell, {Stephen M.} and Pablo Pessacq and Claudio Veloso and McCutcheon, {John P.} and Piotr {\L}ukasik",
note = "Funding Information: Thanks to Ana Maria Marino de Remes Lenicov and her museum staff at Museo de La Plata for facilitating our examination of type specimens in their collections. Thanks to Argentine permitting authorities and Ra{\'u}l Adolfo Pessacq for help with permits and to Iris Peralta of the National University of Cuyo, Mendoza, Argentina for identification of Heterostachys.Thanks to Ivan Nozaic, Kyra Kopestonsky, Sally Beech and Virge Kask for assistance with illustrations.We thank Sean Holland and Kirby Birch at the Center for Anchored Phylogenomics for assistance with data collection and analysis. Thanks to David Marshall, Bert Orr and Heinrich Fliedner for advice on nomenclature and to David Marshall and Kathy Hill and various cicada colleagues around the world (listed by Marshall et al., 2018) for help with collection of non-Derotettix taxa.Thanks to Allen Sanborn for information on D. wagneri localities. David Marshall provided valuable discussion on the systematics and ecology of cicadas,a careful reading of the paper and many excellent suggestions. One anonymous reviewer, Tatiana Ruschel,Masami Hayashi and John A.Allen made helpful suggestions that improved the manuscript. Thanks to the Biodiversity Research Collections, University of Connecticut for housing vouchers of specimens used in this publication. Collection, transportation and export of Derotettix specimens was permitted by the province of Mendoza and the Argentine Ministerio de Ambiente Y Desarrollo Sustentable, Direcci{\'o}n de Fauna Silvestre DSN no. 52460852/18. This research was supported by the National Science Foundation (DEB 1655891 and IOS 1553529), the National Geographic Society grant 9760-15, an Ernst Mayr Travel Grant from the Museum of Comparative Zoology (Harvard University) and several grants from the University of Connecticut. Publisher Copyright: {\textcopyright} 2019 The Linnean Society of London, Biological Journal of the Linnean Society.",
year = "2019",
month = dec,
day = "1",
doi = "10.1093/biolinnean/blz120",
language = "English (US)",
volume = "128",
pages = "865--886",
journal = "Biological Journal of the Linnean Society",
issn = "0024-4066",
publisher = "Wiley-Blackwell",
number = "4",
}