Occurrence and environmental stress responses of two plastid terminal oxidases in Haematococcus pluvialis (Chlorophyceae)

Jiangxin Wang, Milton Sommerfeld, Qiang Hu

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The plastid terminal oxidase (PTOX) is a plastoquinol oxidase involved in carotenoid biosynthesis in higher plants, and may also represent the elusive oxidase in chlororespiration. Haematococcus pluvialis is a green alga that has the ability to synthesize and accumulate large amounts of the red carotenoid astaxanthin (ca. 2% of dry weight) under various stress conditions. However, the occurrence and function of PTOX in astaxanthin synthesis and the stress response in this organism is unknown. In this study, two ptox cDNAs were cloned and sequenced from H. pluvialis and were designated as ptox1 and ptox2. Genome sequence analysis and database searching revealed that duplication of PTOX gene occurred in certain eukaryotic algae, but not in cyanobacteria and higher plants. The physiological and biochemical evidence indicated that PTOX is involved in astaxanthin synthesis and plays a critical protective role against stress. Analysis of the transcriptional expression of the PTOXs and phytoene desaturase gene further suggests that it may be PTOX1 rather than PTOX2 that is co-regulated with astaxanthin synthesis. The fact that the changes in transcripts of ptoxs in response to high light and other stressors and the differential expression of ptox1 and ptox2, suggests that PTOX, coupled with astaxanthin synthesis pathway, exerts broad, yet undefined functions in addition to those identified in higher plants.

Original languageEnglish (US)
Pages (from-to)191-203
Number of pages13
JournalPlanta
Volume230
Issue number1
DOIs
StatePublished - Jun 2009

Fingerprint

Haematococcus pluvialis
Plastids
astaxanthin
Chlorophyceae
plastids
stress response
Oxidoreductases
synthesis
algae
carotenoids
Carotenoids
Chlorophyta
Cyanobacteria
genes
sequence analysis
biosynthesis
Genes
Sequence Analysis
genome
organisms

Keywords

  • Astaxanthin
  • Environmental stress
  • Gene expression
  • Haematococcus pluvialis
  • Plastid terminal oxidase

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Occurrence and environmental stress responses of two plastid terminal oxidases in Haematococcus pluvialis (Chlorophyceae). / Wang, Jiangxin; Sommerfeld, Milton; Hu, Qiang.

In: Planta, Vol. 230, No. 1, 06.2009, p. 191-203.

Research output: Contribution to journalArticle

Wang, Jiangxin ; Sommerfeld, Milton ; Hu, Qiang. / Occurrence and environmental stress responses of two plastid terminal oxidases in Haematococcus pluvialis (Chlorophyceae). In: Planta. 2009 ; Vol. 230, No. 1. pp. 191-203.
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