Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond

Zarraz M.P. Lee, Amisha T. Poret-Peterson, Janet L. Siefert, Drishti Kaul, Ahmed Moustafa, Andrew E. Allen, Chris L. Dupont, Luis E. Eguiarte, Valeria Souza, James Elser

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Nutrient availability and ratios can play an important role in shaping microbial communities of freshwater ecosystems. The Cuatro Ciénegas Basin (CCB) in Mexico is a desert oasis where, perhaps paradoxically, high microbial diversity coincides with extreme oligotrophy. To better understand the effects of nutrients on microbial communities in CCB, a mesocosm experiment was implemented in a stoichiometrically imbalanced pond, Lagunita, which has an average TN:TP ratio of 122 (atomic). The experiment had four treatments, each with five spatial replicates - unamended controls and three fertilization treatments with different nitrogen:phosphorus (N:P) regimes (P only, N:P = 16 and N:P = 75 by atoms). In the water column, quantitative PCR of the 16S rRNA gene indicated that P enrichment alone favored proliferation of bacterial taxa with high rRNA gene copy number, consistent with a previously hypothesized but untested connection between rRNA gene copy number and P requirement. Bacterial and microbial eukaryotic community structure was investigated by pyrosequencing of 16S and 18S rRNA genes from the planktonic and surficial sediment samples. Nutrient enrichment shifted the composition of the planktonic community in a treatment-specific manner and promoted the growth of previously rare bacterial taxa at the expense of the more abundant, potentially endemic, taxa. The eukaryotic community was highly enriched with phototrophic populations in the fertilized treatment. The sediment microbial community exhibited high beta diversity among replicates within treatments, which obscured any changes due to fertilization. Overall, these results showed that nutrient stoichiometry can be an important factor in shaping microbial community structure.

Original languageEnglish (US)
Article number949
JournalFrontiers in Microbiology
Volume8
Issue numberMAY
DOIs
StatePublished - May 30 2017

Fingerprint

rRNA Genes
Food
Gene Dosage
Fertilization
Mexico
Fresh Water
Phosphorus
Ecosystem
Nitrogen
Polymerase Chain Reaction
Water
Growth
Population

Keywords

  • Algae
  • Bacteria
  • Beta diversity
  • Community structure
  • Growth rate hypothesis
  • RRNA gene copy number
  • Stoichiometry

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Lee, Z. M. P., Poret-Peterson, A. T., Siefert, J. L., Kaul, D., Moustafa, A., Allen, A. E., ... Elser, J. (2017). Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond. Frontiers in Microbiology, 8(MAY), [949]. https://doi.org/10.3389/fmicb.2017.00949

Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond. / Lee, Zarraz M.P.; Poret-Peterson, Amisha T.; Siefert, Janet L.; Kaul, Drishti; Moustafa, Ahmed; Allen, Andrew E.; Dupont, Chris L.; Eguiarte, Luis E.; Souza, Valeria; Elser, James.

In: Frontiers in Microbiology, Vol. 8, No. MAY, 949, 30.05.2017.

Research output: Contribution to journalArticle

Lee, ZMP, Poret-Peterson, AT, Siefert, JL, Kaul, D, Moustafa, A, Allen, AE, Dupont, CL, Eguiarte, LE, Souza, V & Elser, J 2017, 'Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond', Frontiers in Microbiology, vol. 8, no. MAY, 949. https://doi.org/10.3389/fmicb.2017.00949
Lee ZMP, Poret-Peterson AT, Siefert JL, Kaul D, Moustafa A, Allen AE et al. Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond. Frontiers in Microbiology. 2017 May 30;8(MAY). 949. https://doi.org/10.3389/fmicb.2017.00949
Lee, Zarraz M.P. ; Poret-Peterson, Amisha T. ; Siefert, Janet L. ; Kaul, Drishti ; Moustafa, Ahmed ; Allen, Andrew E. ; Dupont, Chris L. ; Eguiarte, Luis E. ; Souza, Valeria ; Elser, James. / Nutrient stoichiometry Shapes Microbial Community Structure in an Evaporitic Shallow Pond. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. MAY.
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