Nucleus-to-nucleus gene transfer and protein retargeting into a remnant cytoplasm of cryptophytes and diatoms

Sven B. Gould, Maik S. Sommer, Peter G. Kroth, Gillian Gile, Patrick J. Keeling, Uwe G. Maier

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The complex plastid of the cryptophyte Guillardia theta and of the diatom Phaeodactylum tricornutum can both be traced back to an engulfed eukaryotic red alga. The eukaryotic origin of these plastids is most obvious in cryptophytes, where the organelle still possesses a remnant nucleus, the nucleomorph. The nucleomorph itself is embedded in the periplastid compartment (PPC), the remnant of the former red algal cytosol. In the cryptophyte and diatom, the complex plastid is surrounded by 4 membranes, the outer one being continuous with the host rough endoplasmatic reticulum. In a recent report, we have shown that a nuclear encoded PPC protein of G. theta expressed in P. tricornutum leads to a localization, recently described as being a "bloblike structure," which can be obtained by mutation of plastid protein-targeting sequences of the diatom itself. Here we present further nucleus-encoded PPC proteins from G. theta, such as the eukaryotic translation elongation factor-1α, evidence for their nucleus-to-nucleus gene transfer, and retargeting of the proteins. We also investigated the first nuclear encoded PPC-targeted protein of P. tricornutum (Hsp70) and analyzed it for in vivo localization together with the identified G. theta PPC proteins. This revealed that all localize to the bloblike structures, which we suggest is the highly reduced PPC of P. tricornutum. Furthermore, the described cryptophyte PPC proteins possibly allow the elucidation of the processes by which proteins are involved in different levels of host control over its eukaryotic organelle.

Original languageEnglish (US)
Pages (from-to)2413-2422
Number of pages10
JournalMolecular Biology and Evolution
Volume23
Issue number12
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Gene transfer
Diatoms
Bacillariophyceae
gene transfer
cytoplasm
Cytoplasm
diatom
Phaeodactylum tricornutum
protein
plastid
Plastids
plastids
Proteins
proteins
Organelles
organelles
Chloroplast Proteins
Peptide Elongation Factor 1
Rhodophyta
Reticulum

Keywords

  • Chromists
  • Complex plastids
  • Endosymbiosis
  • Gene transfer
  • Periplastid compartment
  • Protein transport

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Nucleus-to-nucleus gene transfer and protein retargeting into a remnant cytoplasm of cryptophytes and diatoms. / Gould, Sven B.; Sommer, Maik S.; Kroth, Peter G.; Gile, Gillian; Keeling, Patrick J.; Maier, Uwe G.

In: Molecular Biology and Evolution, Vol. 23, No. 12, 12.2006, p. 2413-2422.

Research output: Contribution to journalArticle

Gould, Sven B. ; Sommer, Maik S. ; Kroth, Peter G. ; Gile, Gillian ; Keeling, Patrick J. ; Maier, Uwe G. / Nucleus-to-nucleus gene transfer and protein retargeting into a remnant cytoplasm of cryptophytes and diatoms. In: Molecular Biology and Evolution. 2006 ; Vol. 23, No. 12. pp. 2413-2422.
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