Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli

Robin E. Reed, Madeline F. Baer, Cecilia Guerrier-Takada, Helen Donis-Keller, Sidney Altman

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60% GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.

Original languageEnglish (US)
Pages (from-to)627-636
Number of pages10
JournalCell
Volume30
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Ribonuclease P
Gene encoding
Escherichia coli
Nucleotides
RNA
Genes
Transcription
Transfer RNA
Genetic Promoter Regions
Base Pairing
Plasmids

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli. / Reed, Robin E.; Baer, Madeline F.; Guerrier-Takada, Cecilia; Donis-Keller, Helen; Altman, Sidney.

In: Cell, Vol. 30, No. 2, 1982, p. 627-636.

Research output: Contribution to journalArticle

Reed, Robin E. ; Baer, Madeline F. ; Guerrier-Takada, Cecilia ; Donis-Keller, Helen ; Altman, Sidney. / Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli. In: Cell. 1982 ; Vol. 30, No. 2. pp. 627-636.
@article{0ed85b97f8db49219ef58328517c239b,
title = "Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli",
abstract = "The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60{\%} GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.",
author = "Reed, {Robin E.} and Baer, {Madeline F.} and Cecilia Guerrier-Takada and Helen Donis-Keller and Sidney Altman",
year = "1982",
doi = "10.1016/0092-8674(82)90259-8",
language = "English (US)",
volume = "30",
pages = "627--636",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Nucleotide sequence of the gene encoding the RNA subunit (M1 RNA) of ribonuclease P from Escherichia coli

AU - Reed, Robin E.

AU - Baer, Madeline F.

AU - Guerrier-Takada, Cecilia

AU - Donis-Keller, Helen

AU - Altman, Sidney

PY - 1982

Y1 - 1982

N2 - The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60% GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.

AB - The gene encoding the RNA subunit (M1 RNA) of RNAase P (EC 3.1.26.5) from Escherichia coli has been isolated, and its complete nucleotide sequence, including flanking regions, has been determined. The promoter region, similar to others near genes under stringent control, and the site of transcription termination have been identified. The transcript from the gene (M1 RNA) can be drawn in a secondary structure that has approximately 60% GC base pairs. One hairpin loop of this hypothetical structure has five contiguous nucleotides complementary to invariant nucleotides in the TψCG loop of all E. coli tRNAs. The M1 gene, when subcloned in the plasmid pBR325, can be amplified. It directs production of functional M1 RNA. In an E. coli strain thermosensitive for RNAase P function, the size of the gene transcript is the same as in wild-type E. coli, but less mature M1 RNA is made in the mutant cells.

UR - http://www.scopus.com/inward/record.url?scp=0020184761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020184761&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(82)90259-8

DO - 10.1016/0092-8674(82)90259-8

M3 - Article

C2 - 6183002

AN - SCOPUS:0020184761

VL - 30

SP - 627

EP - 636

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -