Nucleobase-specific enhancement of Cy3 fluorescence

Billie J. Harvey, Marcia Levitus

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

We report on the role of dye-nucleobase interactions on the photophysical properties of the indocarbocyanine Cy3. The fluorescence efficiency and lifetime of Cy3 increase in the presence of all four nucleoside monophosphates. This behavior correlates with an increase in the activation energy for photoisomerization and a ∼4 nm red shift in the fluorescence spectrum. Changes are more dramatic for the purines (dAMP, dGMP) than the pyrimidines (dCMP, dTMP), and for the nonsulfonated cyanine (DiIC 2(3)) than the sulfonated dye (Cy3-SE). These results are consistent with a model in which Cy3-nucleoside π-π interactions decrease the efficiency of photoisomerization, increasing the efficiency of fluorescence.

Original languageEnglish (US)
Pages (from-to)443-448
Number of pages6
JournalJournal of Fluorescence
Volume19
Issue number3
DOIs
StatePublished - May 1 2009

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Keywords

  • Cy dyes
  • Cy3
  • DNA
  • Nucleobases
  • Nucleosides
  • Photoisomerization

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

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