TY - JOUR
T1 - Nucleic Acid Affinity Chromatography
T2 - Preparation and Characterization of Double-Stranded RNA Agarose
AU - Langland, Jeffrey
AU - Pettiford, S. M.
AU - Jacobs, Bertram
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 1995/2
Y1 - 1995/2
N2 - An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl)·poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.
AB - An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl)·poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.
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U2 - 10.1006/prep.1995.1004
DO - 10.1006/prep.1995.1004
M3 - Article
C2 - 7538839
AN - SCOPUS:0029240407
VL - 6
SP - 25
EP - 32
JO - Protein Expression and Purification
JF - Protein Expression and Purification
SN - 1046-5928
IS - 1
ER -