Nucleic Acid Affinity Chromatography: Preparation and Characterization of Double-Stranded RNA Agarose

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27 Scopus citations

Abstract

An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl)·poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.

Original languageEnglish (US)
Pages (from-to)25-32
Number of pages8
JournalProtein Expression and Purification
Volume6
Issue number1
DOIs
StatePublished - Feb 1995

ASJC Scopus subject areas

  • Biotechnology

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