Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas

Hongmiao Sheng, Jinyi Shao, Christopher S. Williams, Michael A. Pereira, Makoto M. Taketo, Masanobu Oshima, Albert B. Reynolds, Mary K. Washington, Raymond N. DuBois, R. Daniel Beauchamp

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Abstract

The physical interaction between β-catenin and the adenomatous polyposis coli (APC) gene, and the ability of APC to regulate cytoplasmic levels of β-catenin suggest a role for β-catenin in colorectal carcinogenesis. In this study, we found that β-catenin immunoreactivity was detected exclusively in the cell membrane and cytoplasm of morphologically normal intestinal epithelial cells with predominant distribution in the differentiated non-proliferative cell population. In contrast, β-catenin was localized predominantly in the nucleus of adenomas from Min/+ mice and transgenic mice expressing a mutant truncated form of the APC gene (Apc(Δ716) mice). β-catenin was expressed predominantly at the cell membrane and cytoplasm of the nontransformed rat intestinal epithelial (RIE-1) cells in culture, whereas predominantly nuclear localization of β-catenin was observed in the human colon cancer cell line SW480. In the azoxymethane (AOM) treated rats, overexpression and nuclear localization of β-catenin was observed in all adenomas. Previous studies have indicated the incidence of APC mutations amongst AOM-induced tumors to be 15% or less. These results demonstrate that nuclear localization of β-catenin is a common event in colorectal tumorigenesis.

Original languageEnglish (US)
Pages (from-to)543-549
Number of pages7
JournalCarcinogenesis
Volume19
Issue number4
DOIs
StatePublished - Apr 1998
Externally publishedYes

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Catenins
Carcinogens
Adenoma
Azoxymethane
APC Genes
Adenomatous Polyposis Coli
Carcinogenesis
Cytoplasm
Cell Membrane
Colonic Neoplasms
Transgenic Mice
Cell Culture Techniques
Epithelial Cells
Cell Line
Mutation

ASJC Scopus subject areas

  • Cancer Research

Cite this

Sheng, H., Shao, J., Williams, C. S., Pereira, M. A., Taketo, M. M., Oshima, M., ... Beauchamp, R. D. (1998). Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas. Carcinogenesis, 19(4), 543-549. https://doi.org/10.1093/carcin/19.4.543

Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas. / Sheng, Hongmiao; Shao, Jinyi; Williams, Christopher S.; Pereira, Michael A.; Taketo, Makoto M.; Oshima, Masanobu; Reynolds, Albert B.; Washington, Mary K.; DuBois, Raymond N.; Beauchamp, R. Daniel.

In: Carcinogenesis, Vol. 19, No. 4, 04.1998, p. 543-549.

Research output: Contribution to journalArticle

Sheng, H, Shao, J, Williams, CS, Pereira, MA, Taketo, MM, Oshima, M, Reynolds, AB, Washington, MK, DuBois, RN & Beauchamp, RD 1998, 'Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas', Carcinogenesis, vol. 19, no. 4, pp. 543-549. https://doi.org/10.1093/carcin/19.4.543
Sheng H, Shao J, Williams CS, Pereira MA, Taketo MM, Oshima M et al. Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas. Carcinogenesis. 1998 Apr;19(4):543-549. https://doi.org/10.1093/carcin/19.4.543
Sheng, Hongmiao ; Shao, Jinyi ; Williams, Christopher S. ; Pereira, Michael A. ; Taketo, Makoto M. ; Oshima, Masanobu ; Reynolds, Albert B. ; Washington, Mary K. ; DuBois, Raymond N. ; Beauchamp, R. Daniel. / Nuclear translocation of β-catenin in hereditary and carcinogen-induced intestinal adenomas. In: Carcinogenesis. 1998 ; Vol. 19, No. 4. pp. 543-549.
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