Abstract
Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes microorganisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1104-1110 |
| Number of pages | 7 |
| Journal | Journal of General Virology |
| Volume | 94 |
| Issue number | PART 5 |
| DOIs | |
| State | Published - May 2013 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Novel ssDNA virus recovered from estuarine mollusc (Amphibola crenata) whose replication associated protein (Rep) shares similarities with Rep-like sequences of bacterial origin. / Dayaram, Anisha; Goldstien, Sharyn; Zawar-Reza, Peyman; Gomez, Christopher; Harding, Jon S.; Varsani, Arvind.
In: Journal of General Virology, Vol. 94, No. PART 5, 05.2013, p. 1104-1110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel ssDNA virus recovered from estuarine mollusc (Amphibola crenata) whose replication associated protein (Rep) shares similarities with Rep-like sequences of bacterial origin
AU - Dayaram, Anisha
AU - Goldstien, Sharyn
AU - Zawar-Reza, Peyman
AU - Gomez, Christopher
AU - Harding, Jon S.
AU - Varsani, Arvind
PY - 2013/5
Y1 - 2013/5
N2 - Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes microorganisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.
AB - Over the past couple of years highly diverse novel ssDNA viruses have been discovered. Here, we present the first ssDNA virus, Gastropod-associated circular ssDNA virus (GaCSV), recovered from a mollusc Amphibola crenata Martyn 1784, which is a deposit feeder that grazes microorganisms and organic detritus on the surface of tidal mudflats. The GaCSV (2351 nt) genome contains two large bidirectionally transcribed ORFs. The smaller ORF (874 nt) has similarities to viral replication-associated protein (Rep) sequences of some bacteria and circoviruses, whereas the larger ORF (955 nt) does not relate to any sequences in public databases and we presume it potentially encodes the capsid protein. Phylogenetic analysis shows that the GaCSV Rep clusters with Rep-like sequences of bacterial origin, highlighting the role of ssDNA viruses in horizontal gene transfer. The occurrence of previously unknown viruses in organisms associated with human pollution is a relatively unexplored field.
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UR - http://www.scopus.com/inward/citedby.url?scp=84876261268&partnerID=8YFLogxK
U2 - 10.1099/vir.0.050088-0
DO - 10.1099/vir.0.050088-0
M3 - Article
C2 - 23364192
AN - SCOPUS:84876261268
VL - 94
SP - 1104
EP - 1110
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - PART 5
ER -