Novel role of surfactant protein A in bacterial sinusitis

Georgios Noutsios, Amanda L. Willis, Julie G. Ledford, Eugene H. Chang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Chronic rhinosinusitis (CRS) is a common inflammatory disorder of the upper airway characterized by chronic inflammation and significant sinonasal remodeling. CRS is comprised of 2 major subgroups, based on whether polyps are present or absent. In some cases, it is characterized by colonization with opportunistic pathogens such as Pseudomonas aeruginosa (PA), Staphylococcus aureus, and other bacteria. The innate immune system of the sinonasal epithelium is the first line of defense against inhaled pathogens. Surfactant protein A (SP-A) is a member of the collectin family secreted by the airway epithelia and plays a critical role in airway innate immunity, as it can aggregate bacteria. We hypothesized that SP-A plays a role in bacterial CRS. Methods: Air-liquid interface (ALI) cultures of nasal epithelial cells were derived from human ex-vivo healthy and CRS sinus tissues (n = 26) and challenged with PA. SP-A levels were measured with western blot and quantitative reverse transcript-polymerase chain reaction (qRT-PCR) in ALI and sinus tissues. Results: We determined that SP-A: (i) mRNA and protein levels are increased significantly in CRS tissues compared with healthy sinuses; (ii) although primarily expressed in the lung, it is also synthesized and expressed in sinonasal epithelia; (ii) is expressed in the sinuses of an SP-A humanized transgenic mouse but not in SP-A knockout mice; (iv) mRNA levels are upregulated significantly during PA challenge, but protein levels are downregulated 4 hours postchallenge and upregulated at 12 hours. Conclusion: Our data suggest that SP-A is expressed in the sinuses and that it plays a role in the sinus innate immune responses during bacterial infections.

Original languageEnglish (US)
Pages (from-to)897-903
Number of pages7
JournalInternational Forum of Allergy and Rhinology
Volume7
Issue number9
DOIs
StatePublished - Sep 1 2017
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein A
Sinusitis
Pseudomonas aeruginosa
Epithelium
Innate Immunity
Collectins
Air
Bacteria
Messenger RNA
Polyps
Nose
Bacterial Infections
Knockout Mice
Transgenic Mice
Staphylococcus aureus
Immune System
Proteins
Down-Regulation
Western Blotting
Epithelial Cells

Keywords

  • CRS
  • innate immunity, Pseudomonas aeruginosa, rhinosinusitis, SP-A

ASJC Scopus subject areas

  • Immunology and Allergy
  • Otorhinolaryngology

Cite this

Novel role of surfactant protein A in bacterial sinusitis. / Noutsios, Georgios; Willis, Amanda L.; Ledford, Julie G.; Chang, Eugene H.

In: International Forum of Allergy and Rhinology, Vol. 7, No. 9, 01.09.2017, p. 897-903.

Research output: Contribution to journalArticle

Noutsios, Georgios ; Willis, Amanda L. ; Ledford, Julie G. ; Chang, Eugene H. / Novel role of surfactant protein A in bacterial sinusitis. In: International Forum of Allergy and Rhinology. 2017 ; Vol. 7, No. 9. pp. 897-903.
@article{0a37dd762dc0426c915a23b4af3050bc,
title = "Novel role of surfactant protein A in bacterial sinusitis",
abstract = "Background: Chronic rhinosinusitis (CRS) is a common inflammatory disorder of the upper airway characterized by chronic inflammation and significant sinonasal remodeling. CRS is comprised of 2 major subgroups, based on whether polyps are present or absent. In some cases, it is characterized by colonization with opportunistic pathogens such as Pseudomonas aeruginosa (PA), Staphylococcus aureus, and other bacteria. The innate immune system of the sinonasal epithelium is the first line of defense against inhaled pathogens. Surfactant protein A (SP-A) is a member of the collectin family secreted by the airway epithelia and plays a critical role in airway innate immunity, as it can aggregate bacteria. We hypothesized that SP-A plays a role in bacterial CRS. Methods: Air-liquid interface (ALI) cultures of nasal epithelial cells were derived from human ex-vivo healthy and CRS sinus tissues (n = 26) and challenged with PA. SP-A levels were measured with western blot and quantitative reverse transcript-polymerase chain reaction (qRT-PCR) in ALI and sinus tissues. Results: We determined that SP-A: (i) mRNA and protein levels are increased significantly in CRS tissues compared with healthy sinuses; (ii) although primarily expressed in the lung, it is also synthesized and expressed in sinonasal epithelia; (ii) is expressed in the sinuses of an SP-A humanized transgenic mouse but not in SP-A knockout mice; (iv) mRNA levels are upregulated significantly during PA challenge, but protein levels are downregulated 4 hours postchallenge and upregulated at 12 hours. Conclusion: Our data suggest that SP-A is expressed in the sinuses and that it plays a role in the sinus innate immune responses during bacterial infections.",
keywords = "CRS, innate immunity, Pseudomonas aeruginosa, rhinosinusitis, SP-A",
author = "Georgios Noutsios and Willis, {Amanda L.} and Ledford, {Julie G.} and Chang, {Eugene H.}",
year = "2017",
month = "9",
day = "1",
doi = "10.1002/alr.21985",
language = "English (US)",
volume = "7",
pages = "897--903",
journal = "International Forum of Allergy and Rhinology",
issn = "2042-6976",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Novel role of surfactant protein A in bacterial sinusitis

AU - Noutsios, Georgios

AU - Willis, Amanda L.

AU - Ledford, Julie G.

AU - Chang, Eugene H.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Background: Chronic rhinosinusitis (CRS) is a common inflammatory disorder of the upper airway characterized by chronic inflammation and significant sinonasal remodeling. CRS is comprised of 2 major subgroups, based on whether polyps are present or absent. In some cases, it is characterized by colonization with opportunistic pathogens such as Pseudomonas aeruginosa (PA), Staphylococcus aureus, and other bacteria. The innate immune system of the sinonasal epithelium is the first line of defense against inhaled pathogens. Surfactant protein A (SP-A) is a member of the collectin family secreted by the airway epithelia and plays a critical role in airway innate immunity, as it can aggregate bacteria. We hypothesized that SP-A plays a role in bacterial CRS. Methods: Air-liquid interface (ALI) cultures of nasal epithelial cells were derived from human ex-vivo healthy and CRS sinus tissues (n = 26) and challenged with PA. SP-A levels were measured with western blot and quantitative reverse transcript-polymerase chain reaction (qRT-PCR) in ALI and sinus tissues. Results: We determined that SP-A: (i) mRNA and protein levels are increased significantly in CRS tissues compared with healthy sinuses; (ii) although primarily expressed in the lung, it is also synthesized and expressed in sinonasal epithelia; (ii) is expressed in the sinuses of an SP-A humanized transgenic mouse but not in SP-A knockout mice; (iv) mRNA levels are upregulated significantly during PA challenge, but protein levels are downregulated 4 hours postchallenge and upregulated at 12 hours. Conclusion: Our data suggest that SP-A is expressed in the sinuses and that it plays a role in the sinus innate immune responses during bacterial infections.

AB - Background: Chronic rhinosinusitis (CRS) is a common inflammatory disorder of the upper airway characterized by chronic inflammation and significant sinonasal remodeling. CRS is comprised of 2 major subgroups, based on whether polyps are present or absent. In some cases, it is characterized by colonization with opportunistic pathogens such as Pseudomonas aeruginosa (PA), Staphylococcus aureus, and other bacteria. The innate immune system of the sinonasal epithelium is the first line of defense against inhaled pathogens. Surfactant protein A (SP-A) is a member of the collectin family secreted by the airway epithelia and plays a critical role in airway innate immunity, as it can aggregate bacteria. We hypothesized that SP-A plays a role in bacterial CRS. Methods: Air-liquid interface (ALI) cultures of nasal epithelial cells were derived from human ex-vivo healthy and CRS sinus tissues (n = 26) and challenged with PA. SP-A levels were measured with western blot and quantitative reverse transcript-polymerase chain reaction (qRT-PCR) in ALI and sinus tissues. Results: We determined that SP-A: (i) mRNA and protein levels are increased significantly in CRS tissues compared with healthy sinuses; (ii) although primarily expressed in the lung, it is also synthesized and expressed in sinonasal epithelia; (ii) is expressed in the sinuses of an SP-A humanized transgenic mouse but not in SP-A knockout mice; (iv) mRNA levels are upregulated significantly during PA challenge, but protein levels are downregulated 4 hours postchallenge and upregulated at 12 hours. Conclusion: Our data suggest that SP-A is expressed in the sinuses and that it plays a role in the sinus innate immune responses during bacterial infections.

KW - CRS

KW - innate immunity, Pseudomonas aeruginosa, rhinosinusitis, SP-A

UR - http://www.scopus.com/inward/record.url?scp=85025432072&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025432072&partnerID=8YFLogxK

U2 - 10.1002/alr.21985

DO - 10.1002/alr.21985

M3 - Article

VL - 7

SP - 897

EP - 903

JO - International Forum of Allergy and Rhinology

JF - International Forum of Allergy and Rhinology

SN - 2042-6976

IS - 9

ER -