Novel Rapid Method of Gene & Cell Selection For Research & Healthcare Applications

Neal Woodbury (Inventor)

Research output: Patent

Abstract

Directed evolution is a process wherein a gene sequence is varied randomly, generating a library of mutated genes. These mutated genes are expressed and the functions of those gene products are assayed. A selection procedure is then applied to select those genes that express products with desirable functions. These genes are then selectively amplified and the mutagenesis, screening and selection process is repeated until gene products with the most desirable functions are obtained. The screening process involves exciting genes with light and observing the fluorescence from these genes or from molecules they are associated with. This screening process, being visual, is slow, unautomated and incompatible with high throughput screening. This greatly limits the number of cells that can be selected. It is almost always the case that directed evolution procedures select for changes in either the amount of or the chemical properties of the gene, but never both. Thus the capability of performing directed evolution using a fluorescent assay that was both amenable to automation and would distinguish between fluorescence amplitude and fluorescence lifetime is a significant asset not only for research but for diagnostics and therapeutics as well. Neal Woodbury at Arizona State University has invented a method using fluorescence and optical techniques for screening large numbers of individual cells or colonies of cells that yield fluorescence lifetime data. Analysis of this data is controlled and performed in an automated and rapid manner. This screening method can then be used in cell selection procedures in conjunction with additional methodologies based on their fluorescent properties, which were also developed by the research group. Using these new methods, one can kill cells in a uniquely selective manner, thus promoting the selective survival of the remaining cells. Potential Applications Biotechnology research and development Cell selection Directed evolutionBenefits and Advantages Automated process Rapid screening of a large number of colonies Highly selectiveDowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Woodbury's departmental webpageDr. Woodbury's directory webpage
Original languageEnglish (US)
StatePublished - Jun 13 2000

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Delivery of Health Care
Research
Genes
Fluorescence
Cell Count
Inventors
Therapeutic Human Experimentation
Directories
Automation
Biotechnology
Gene Library
Mutagenesis
Cell Survival
Light

Cite this

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title = "Novel Rapid Method of Gene & Cell Selection For Research & Healthcare Applications",
abstract = "Directed evolution is a process wherein a gene sequence is varied randomly, generating a library of mutated genes. These mutated genes are expressed and the functions of those gene products are assayed. A selection procedure is then applied to select those genes that express products with desirable functions. These genes are then selectively amplified and the mutagenesis, screening and selection process is repeated until gene products with the most desirable functions are obtained. The screening process involves exciting genes with light and observing the fluorescence from these genes or from molecules they are associated with. This screening process, being visual, is slow, unautomated and incompatible with high throughput screening. This greatly limits the number of cells that can be selected. It is almost always the case that directed evolution procedures select for changes in either the amount of or the chemical properties of the gene, but never both. Thus the capability of performing directed evolution using a fluorescent assay that was both amenable to automation and would distinguish between fluorescence amplitude and fluorescence lifetime is a significant asset not only for research but for diagnostics and therapeutics as well. Neal Woodbury at Arizona State University has invented a method using fluorescence and optical techniques for screening large numbers of individual cells or colonies of cells that yield fluorescence lifetime data. Analysis of this data is controlled and performed in an automated and rapid manner. This screening method can then be used in cell selection procedures in conjunction with additional methodologies based on their fluorescent properties, which were also developed by the research group. Using these new methods, one can kill cells in a uniquely selective manner, thus promoting the selective survival of the remaining cells. Potential Applications Biotechnology research and development Cell selection Directed evolutionBenefits and Advantages Automated process Rapid screening of a large number of colonies Highly selectiveDowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Woodbury's departmental webpageDr. Woodbury's directory webpage",
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N2 - Directed evolution is a process wherein a gene sequence is varied randomly, generating a library of mutated genes. These mutated genes are expressed and the functions of those gene products are assayed. A selection procedure is then applied to select those genes that express products with desirable functions. These genes are then selectively amplified and the mutagenesis, screening and selection process is repeated until gene products with the most desirable functions are obtained. The screening process involves exciting genes with light and observing the fluorescence from these genes or from molecules they are associated with. This screening process, being visual, is slow, unautomated and incompatible with high throughput screening. This greatly limits the number of cells that can be selected. It is almost always the case that directed evolution procedures select for changes in either the amount of or the chemical properties of the gene, but never both. Thus the capability of performing directed evolution using a fluorescent assay that was both amenable to automation and would distinguish between fluorescence amplitude and fluorescence lifetime is a significant asset not only for research but for diagnostics and therapeutics as well. Neal Woodbury at Arizona State University has invented a method using fluorescence and optical techniques for screening large numbers of individual cells or colonies of cells that yield fluorescence lifetime data. Analysis of this data is controlled and performed in an automated and rapid manner. This screening method can then be used in cell selection procedures in conjunction with additional methodologies based on their fluorescent properties, which were also developed by the research group. Using these new methods, one can kill cells in a uniquely selective manner, thus promoting the selective survival of the remaining cells. Potential Applications Biotechnology research and development Cell selection Directed evolutionBenefits and Advantages Automated process Rapid screening of a large number of colonies Highly selectiveDowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Woodbury's departmental webpageDr. Woodbury's directory webpage

AB - Directed evolution is a process wherein a gene sequence is varied randomly, generating a library of mutated genes. These mutated genes are expressed and the functions of those gene products are assayed. A selection procedure is then applied to select those genes that express products with desirable functions. These genes are then selectively amplified and the mutagenesis, screening and selection process is repeated until gene products with the most desirable functions are obtained. The screening process involves exciting genes with light and observing the fluorescence from these genes or from molecules they are associated with. This screening process, being visual, is slow, unautomated and incompatible with high throughput screening. This greatly limits the number of cells that can be selected. It is almost always the case that directed evolution procedures select for changes in either the amount of or the chemical properties of the gene, but never both. Thus the capability of performing directed evolution using a fluorescent assay that was both amenable to automation and would distinguish between fluorescence amplitude and fluorescence lifetime is a significant asset not only for research but for diagnostics and therapeutics as well. Neal Woodbury at Arizona State University has invented a method using fluorescence and optical techniques for screening large numbers of individual cells or colonies of cells that yield fluorescence lifetime data. Analysis of this data is controlled and performed in an automated and rapid manner. This screening method can then be used in cell selection procedures in conjunction with additional methodologies based on their fluorescent properties, which were also developed by the research group. Using these new methods, one can kill cells in a uniquely selective manner, thus promoting the selective survival of the remaining cells. Potential Applications Biotechnology research and development Cell selection Directed evolutionBenefits and Advantages Automated process Rapid screening of a large number of colonies Highly selectiveDowload Original PDFFor more information about the inventor(s) and their research, please see Dr. Woodbury's departmental webpageDr. Woodbury's directory webpage

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