Novel quantitative biosystem for modeling physiological fluid shear stress on cells

Eric A. Nauman, C. Mark Ott, Ed Sander, Don L. Tucker, Duane Pierson, James W. Wilson, Cheryl Nickerson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen Salmonella enterica serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.

Original languageEnglish (US)
Pages (from-to)699-705
Number of pages7
JournalApplied and Environmental Microbiology
Volume73
Issue number3
DOIs
StatePublished - Feb 2007

Fingerprint

shear stress
Host-Pathogen Interactions
Batch Cell Culture Techniques
Salmonella enterica
fluid
Bioreactors
Growth
modeling
Theoretical Models
Vaccines
Cell Culture Techniques
Cell Count
cells
pathogen
Infection
microorganisms
pathogens
Proteins
host-pathogen interaction
microbial genetics

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Novel quantitative biosystem for modeling physiological fluid shear stress on cells. / Nauman, Eric A.; Ott, C. Mark; Sander, Ed; Tucker, Don L.; Pierson, Duane; Wilson, James W.; Nickerson, Cheryl.

In: Applied and Environmental Microbiology, Vol. 73, No. 3, 02.2007, p. 699-705.

Research output: Contribution to journalArticle

Nauman, Eric A. ; Ott, C. Mark ; Sander, Ed ; Tucker, Don L. ; Pierson, Duane ; Wilson, James W. ; Nickerson, Cheryl. / Novel quantitative biosystem for modeling physiological fluid shear stress on cells. In: Applied and Environmental Microbiology. 2007 ; Vol. 73, No. 3. pp. 699-705.
@article{b3b038d2e2e94d5b9b5326cbc4cbe4c7,
title = "Novel quantitative biosystem for modeling physiological fluid shear stress on cells",
abstract = "The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen Salmonella enterica serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.",
author = "Nauman, {Eric A.} and Ott, {C. Mark} and Ed Sander and Tucker, {Don L.} and Duane Pierson and Wilson, {James W.} and Cheryl Nickerson",
year = "2007",
month = "2",
doi = "10.1128/AEM.02428-06",
language = "English (US)",
volume = "73",
pages = "699--705",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Novel quantitative biosystem for modeling physiological fluid shear stress on cells

AU - Nauman, Eric A.

AU - Ott, C. Mark

AU - Sander, Ed

AU - Tucker, Don L.

AU - Pierson, Duane

AU - Wilson, James W.

AU - Nickerson, Cheryl

PY - 2007/2

Y1 - 2007/2

N2 - The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen Salmonella enterica serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.

AB - The response of microbes to changes in the mechanical force of fluid shear has important implications for pathogens, which experience wide fluctuations in fluid shear in vivo during infection. However, the majority of studies have not cultured microbes under physiological fluid shear conditions within a range commonly encountered by microbes during host-pathogen interactions. Here we describe a convenient batch culture biosystem in which (i) the levels of fluid shear force can be varied within physiologically relevant ranges and quantified via mathematical models and (ii) large numbers of cells can be planktonically grown and harvested to examine the effect of fluid shear levels on microbial genomic and phenotypic responses. A quantitative model based on numerical simulations and in situ imaging analysis was developed to calculate the fluid shear imparted by spherical beads of different sizes on bacterial cell cultures grown in a rotating wall vessel (RWV) bioreactor. To demonstrate the application of this model, we subjected cultures of the bacterial pathogen Salmonella enterica serovar Typhimurium to three physiologically-relevant fluid shear ranges during growth in the RVW and demonstrated a progressive relationship between the applied fluid shear and the bacterial genetic and phenotypic responses. By applying this model to different cell types, including other bacterial pathogens, entire classes of genes and proteins involved in cellular interactions may be discovered that have not previously been identified during growth under conventional culture conditions, leading to new targets for vaccine and therapeutic development.

UR - http://www.scopus.com/inward/record.url?scp=33846930603&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846930603&partnerID=8YFLogxK

U2 - 10.1128/AEM.02428-06

DO - 10.1128/AEM.02428-06

M3 - Article

C2 - 17142365

AN - SCOPUS:33846930603

VL - 73

SP - 699

EP - 705

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 3

ER -