TY - JOUR
T1 - Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12
AU - Oppmann, Birgit
AU - Lesley, Robin
AU - Blom, Bianca
AU - Timans, Jackie C.
AU - Xu, Yuming
AU - Hunte, Brisdell
AU - Vega, Felix
AU - Yu, Nancy
AU - Wang, Jing
AU - Singh, Komal
AU - Zonin, Francesca
AU - Vaisberg, Elena
AU - Churakova, Tatyana
AU - Liu, Man ru
AU - Gorman, Daniel
AU - Wagner, Janet
AU - Zurawski, Sandra
AU - Liu, Yong Jun
AU - Abrams, John S.
AU - Moore, Kevin W.
AU - Rennick, Donna
AU - De Waal-Malefyt, Rene
AU - Hannum, Charles
AU - Bazan, J. Fernando
AU - Kastelein, Robert A.
N1 - Funding Information:
We thank Deborah Ligett and Sylvia Lo for synthesizing oligonucleotides, Terri McClanahan for providing cDNA libraries, Steve Ho, Svetlana Antonenko, and Thomas J. Cradick for technical support, and Gary Burget and Maribel Andonian for graphics help. DNAX is supported by Schering Plough.
PY - 2000/11
Y1 - 2000/11
N2 - A novel sequence discovered in a computational screen appears distantly related to the p35 subunit of IL-12. This factor, which we term p19, shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a novel, biologically active, composite cytokine, which we term IL-23. Activated dendritic cells secrete detectable levels of this complex. IL-23 binds to IL-12Rβ1 but fails to engage IL-12Rβ2; nonetheless, IL-23 activates Stat4 in PHA blast T cells. IL-23 induces strong proliferation of mouse memory (CD4+CD45Rb(low)) T cells, a unique activity of IL-23 as IL-12 has no effect on this cell population. Similar to IL-12, human IL-23 stimulates IFN-γ production and proliferation in PHA blast T cells, as well as in CD45RO (memory) T cells.
AB - A novel sequence discovered in a computational screen appears distantly related to the p35 subunit of IL-12. This factor, which we term p19, shows no biological activity by itself; instead, it combines with the p40 subunit of IL-12 to form a novel, biologically active, composite cytokine, which we term IL-23. Activated dendritic cells secrete detectable levels of this complex. IL-23 binds to IL-12Rβ1 but fails to engage IL-12Rβ2; nonetheless, IL-23 activates Stat4 in PHA blast T cells. IL-23 induces strong proliferation of mouse memory (CD4+CD45Rb(low)) T cells, a unique activity of IL-23 as IL-12 has no effect on this cell population. Similar to IL-12, human IL-23 stimulates IFN-γ production and proliferation in PHA blast T cells, as well as in CD45RO (memory) T cells.
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U2 - 10.1016/S1074-7613(00)00070-4
DO - 10.1016/S1074-7613(00)00070-4
M3 - Article
C2 - 11114383
AN - SCOPUS:0033638507
SN - 1074-7613
VL - 13
SP - 715
EP - 725
JO - Immunity
JF - Immunity
IS - 5
ER -