This invention presents a novel methodology to generate biosensors using aptamers with fluorescent DNA base analogs. Existing aptamer technology allows generation of specific aptamers for nearly any analyte of interest. Extension of this technology to create fluorescent aptamers provide with an ability to generate any biosensor. When combined with other existing technologies to make DNA microarrays, this new approach could be used for manufacutre of high-throughput detection systems. This technology could also be used to generate novel imaging agents for in vivo biological imaging. The main advantage of this invention over previously presented approaches is that it generates fluorescence signal, which is binding specific, and generated signals have much better signal-to-noise levels.
|Original language||English (US)|
|State||Published - Aug 10 2004|