Novel high-throughput screening system for cancer therapy with simultaneous combination treatments

J. Y. Kim, D. Taylor, Kaushal Rege, H. S. Kim, A. R. Han, A. Jayaraman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this paper, we report the development of a microfluidic high-throughput screening system for high-throughput screening of drug molecule interactions on cancer cell death. A key feature is that 64 pair-wise concentrations (between eight concentrations of two drugs) are generated using a traditional diffusive microfluidic mixer and a time-dependent variable concentration mixer. Using this novel high-throughput combination array, we generated pair-wise combinations of the cancer drug sensitizer doxorubicin and the cytokine TRAIL, and used it for on-chip screening against PC3 prostate cancer cells. Our results identify a concentration range where doxorubicin and TRAIL are synergistic and demonstrates the utility of the system as a high-throughput anti-cancer drug screening tool.

Original languageEnglish (US)
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages836-838
Number of pages3
Volume2
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/3/1010/7/10

Fingerprint

Screening
Throughput
Microfluidics
Cell death
Cells
Molecules

Keywords

  • Combination treatment
  • Doxorubicin
  • High-throughput screening
  • Prostate cancer
  • TRAIL

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Kim, J. Y., Taylor, D., Rege, K., Kim, H. S., Han, A. R., & Jayaraman, A. (2010). Novel high-throughput screening system for cancer therapy with simultaneous combination treatments. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 2, pp. 836-838)

Novel high-throughput screening system for cancer therapy with simultaneous combination treatments. / Kim, J. Y.; Taylor, D.; Rege, Kaushal; Kim, H. S.; Han, A. R.; Jayaraman, A.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. p. 836-838.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kim, JY, Taylor, D, Rege, K, Kim, HS, Han, AR & Jayaraman, A 2010, Novel high-throughput screening system for cancer therapy with simultaneous combination treatments. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 2, pp. 836-838, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/3/10.
Kim JY, Taylor D, Rege K, Kim HS, Han AR, Jayaraman A. Novel high-throughput screening system for cancer therapy with simultaneous combination treatments. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2. 2010. p. 836-838
Kim, J. Y. ; Taylor, D. ; Rege, Kaushal ; Kim, H. S. ; Han, A. R. ; Jayaraman, A. / Novel high-throughput screening system for cancer therapy with simultaneous combination treatments. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. pp. 836-838
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