Novel fungal FAD glucose dehydrogenase derived from Aspergillus niger for glucose enzyme sensor strips

Koji Sode, Noya Loew, Yosuke Ohnishi, Hayato Tsuruta, Kazushige Mori, Katsuhiro Kojima, Wakako Tsugawa, Jeffrey LaBelle, David C. Klonoff

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

In this study, a novel fungus FAD dependent glucose dehydrogenase, derived from Aspergillus niger (AnGDH), was characterized. This enzyme's potential for the use as the enzyme for blood glucose monitor enzyme sensor strips was evaluated, especially by investigating the effect of the presence of xylose during glucose measurements. The substrate specificity of AnGDH towards glucose was investigated, and only xylose was found as a competing substrate. The specific catalytic efficiency for xylose compared to glucose was 1.8%. The specific activity of AnGDH for xylose at 5 mM concentration compared to glucose was 3.5%. No other sugars were used as substrate by this enzyme. The superior substrate specificity of AnGDH was also demonstrated in the performance of enzyme sensor strips. The impact of spiking xylose in a sample with physiological glucose concentrations on the sensor signals was investigated, and it was found that enzyme sensor strips using AnGDH were not affected at all by 5 mM (75 mg/dL) xylose. This is the first report of an enzyme sensor strip using a fungus derived FADGDH, which did not show any positive bias at a therapeutic level xylose concentration on the signal for a glucose sample. This clearly indicates the superiority of AnGDH over other conventionally used fungi derived FADGDHs in the application for SMBG sensor strips. The negligible activity of AnGDH towards xylose was also explained on the basis of a 3D structural model, which was compared to the 3D structures of A. flavus derived FADGDH and of two glucose oxidases.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalBiosensors and Bioelectronics
Volume87
DOIs
StatePublished - Jan 15 2017

Keywords

  • Aspergillus flavus
  • Aspergillus niger
  • BG Monitor
  • FAD-dependent glucose dehydrogenase
  • Glucose specificity
  • Xylose

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Medicine(all)
  • Biomedical Engineering
  • Electrochemistry

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    Sode, K., Loew, N., Ohnishi, Y., Tsuruta, H., Mori, K., Kojima, K., Tsugawa, W., LaBelle, J., & Klonoff, D. C. (2017). Novel fungal FAD glucose dehydrogenase derived from Aspergillus niger for glucose enzyme sensor strips. Biosensors and Bioelectronics, 87, 305-311. https://doi.org/10.1016/j.bios.2016.08.053