Abstract
Advances in molecular techniques used in viral metagenomics coupled with high throughput sequencing is rapidly expanding our knowledge of plant-associated virus diversity. Applying such approaches, we have identified five novel circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses from Poaceae and Apiaceae plant from South Africa and New Zealand. These viruses have a simple genomic organization, including two open reading frames that likely encode a Rep and a capsid protein (CP), a conserved nonanucleotide motif on the apex of a putative stem loop structure, and conserved rolling-circle replication and helicase motifs within their likely Rep: all suggesting that they replicate through rolling-circle replication. The Reps and the CPs putatively encoded by these five novel viruses share low to moderate degrees of similarity (22.1 - 44.6%) with other CRESS DNA viruses.
| Original language | English (US) |
|---|---|
| Journal | Archives of Virology |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Novel circular DNA viruses associated with Apiaceae and Poaceae from South Africa and New Zealand. / Richet, Cécile; Kraberger, Simona; Filloux, Denis; Bernardo, Pauline; Harkins, Gordon W.; Martin, Darren P.; Roumagnac, Philippe; Varsani, Arvind.
In: Archives of Virology, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel circular DNA viruses associated with Apiaceae and Poaceae from South Africa and New Zealand
AU - Richet, Cécile
AU - Kraberger, Simona
AU - Filloux, Denis
AU - Bernardo, Pauline
AU - Harkins, Gordon W.
AU - Martin, Darren P.
AU - Roumagnac, Philippe
AU - Varsani, Arvind
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Advances in molecular techniques used in viral metagenomics coupled with high throughput sequencing is rapidly expanding our knowledge of plant-associated virus diversity. Applying such approaches, we have identified five novel circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses from Poaceae and Apiaceae plant from South Africa and New Zealand. These viruses have a simple genomic organization, including two open reading frames that likely encode a Rep and a capsid protein (CP), a conserved nonanucleotide motif on the apex of a putative stem loop structure, and conserved rolling-circle replication and helicase motifs within their likely Rep: all suggesting that they replicate through rolling-circle replication. The Reps and the CPs putatively encoded by these five novel viruses share low to moderate degrees of similarity (22.1 - 44.6%) with other CRESS DNA viruses.
AB - Advances in molecular techniques used in viral metagenomics coupled with high throughput sequencing is rapidly expanding our knowledge of plant-associated virus diversity. Applying such approaches, we have identified five novel circular replication-associated protein (Rep)-encoding single-stranded (CRESS) DNA viruses from Poaceae and Apiaceae plant from South Africa and New Zealand. These viruses have a simple genomic organization, including two open reading frames that likely encode a Rep and a capsid protein (CP), a conserved nonanucleotide motif on the apex of a putative stem loop structure, and conserved rolling-circle replication and helicase motifs within their likely Rep: all suggesting that they replicate through rolling-circle replication. The Reps and the CPs putatively encoded by these five novel viruses share low to moderate degrees of similarity (22.1 - 44.6%) with other CRESS DNA viruses.
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UR - http://www.scopus.com/inward/citedby.url?scp=85053438130&partnerID=8YFLogxK
U2 - 10.1007/s00705-018-4031-3
DO - 10.1007/s00705-018-4031-3
M3 - Article
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
ER -