Abstract

A simple device for high-speed microfluidic delivery of liquid samples to a surface plasmon resonance sensor surface is presented. The delivery platform is comprised of a four-port microfluidic cell, two ports serve as inlets for buffer and sample solutions, respectively, and a high-speed selector valve to control the alternate opening and closing of the two outlet ports. The time scale of buffer/sample switching (or sample injection rise and fall time) is on the order of milliseconds, thereby minimizing the opportunity for sample plug dispersion. The high rates of mass transport to and from the central microfluidic sensing region allow for SPR-based kinetic analysis of binding events with dissociation rate constants (kd) up to 130 s -1. The required sample volume is only 1 μL, allowing for minimal sample consumption during high-speed kinetic binding measurement.

Original languageEnglish (US)
Article number106110
JournalReview of Scientific Instruments
Volume84
Issue number10
DOIs
StatePublished - Oct 2013

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Microfluidics
Buffers
cells
Kinetics
Surface Plasmon Resonance
Plant shutdowns
Surface plasmon resonance
high speed
Rate constants
Mass transfer
delivery
buffers
Equipment and Supplies
Injections
Sensors
Liquids
selectors
kinetics
closing
plugs

ASJC Scopus subject areas

  • Instrumentation
  • Medicine(all)

Cite this

Note : Four-port microfluidic flow-cell with instant sample switching. / Macgriff, Christopher A.; Wang, Shaopeng; Tao, Nongjian.

In: Review of Scientific Instruments, Vol. 84, No. 10, 106110, 10.2013.

Research output: Contribution to journalArticle

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