Noise reduction method for quantifying nanoparticle light scattering in low magnification dark-field microscope far-field images

Dali Sun, Jia Fan, Chang Liu, Yang Liu, Yang Bu, Christopher J. Lyon, Ye Hu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nanoparticles have become a powerful tool for cell imaging and biomolecule, cell and protein interaction studies, but are difficult to rapidly and accurately measure in most assays. Dark-field microscope (DFM) image analysis approaches used to quantify nanoparticles require highmagnification near-field (HN) images that are labor intensive due to a requirement for manual image selection and focal adjustments needed when identifying and capturing new regions of interest. Low-magnification far-field (LF) DFM imagery is technically simpler to perform but cannot be used as an alternate to HN-DFM quantification, since it is highly sensitive to surface artifacts and debris that can easily mask nanoparticle signal. We now describe a new noise reduction approach that markedly reduces LF-DFM image artifacts to allow sensitive and accurate nanoparticle signal quantification from LF-DFM images. We have used this approach to develop a "Dark Scatter Master" (DSM) algorithm for the popular NIH image analysis program ImageJ, which can be readily adapted for use with automated high-throughput assay analyses. This method demonstrated robust performance quantifying nanoparticles in different assay formats, including a novel method that quantified extracellular vesicles in patient blood sample to detect pancreatic cancer cases. Based on these results, we believe our LF-DFM quantification method can markedly decrease the analysis time of most nanoparticle-based assays to impact both basic research and clinical analyses.

Original languageEnglish (US)
Pages (from-to)12001-12005
Number of pages5
JournalAnalytical Chemistry
Volume88
Issue number24
DOIs
StatePublished - Jan 1 2016

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Noise abatement
Light scattering
Microscopes
Nanoparticles
Assays
Image analysis
Biomolecules
Debris
Masks
Blood
Throughput
Personnel
Imaging techniques
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Noise reduction method for quantifying nanoparticle light scattering in low magnification dark-field microscope far-field images. / Sun, Dali; Fan, Jia; Liu, Chang; Liu, Yang; Bu, Yang; Lyon, Christopher J.; Hu, Ye.

In: Analytical Chemistry, Vol. 88, No. 24, 01.01.2016, p. 12001-12005.

Research output: Contribution to journalArticle

Sun, Dali ; Fan, Jia ; Liu, Chang ; Liu, Yang ; Bu, Yang ; Lyon, Christopher J. ; Hu, Ye. / Noise reduction method for quantifying nanoparticle light scattering in low magnification dark-field microscope far-field images. In: Analytical Chemistry. 2016 ; Vol. 88, No. 24. pp. 12001-12005.
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