Nitrogen transfer in the arbuscular mycorrhizal symbiosis

Manjula Govindarajulu, Philip E. Pfeffer, Hairu Jin, Jehad Abubaker, David D. Douds, James W. Allen, Heike Bücking, Peter Lammers, Yair Shachar-Hill

Research output: Contribution to journalArticle

509 Citations (Scopus)

Abstract

Most land plants are symbiotic with arbuscular mycorrhizal fungi (AMF), which take up mineral nutrients from the soil and exchange them with plants for photosynthetically fixed carbon. This exchange is a significant factor in global nutrient cycles as well as in the ecology, evolution and physiology of plants. Despite its importance as a nutrient, very little is known about how AMF take up nitrogen and transfer it to their host plants. Here we report the results of stable isotope labelling experiments showing that inorganic nitrogen taken up by the fungus outside the roots is incorporated into amino acids, translocated from the extraradical to the intraradical mycelium as arginine, but transferred to the plant without carbon. Consistent with this mechanism, the genes of primary nitrogen assimilation are preferentially expressed in the extraradical tissues, whereas genes associated with arginine breakdown are more highly expressed in the intraradical mycelium. Strong changes in the expression of these genes in response to nitrogen availability and form also support the operation of this novel metabolic pathway in the arbuscular mycorrhizal symbiosis.

Original languageEnglish (US)
Pages (from-to)819-823
Number of pages5
JournalNature
Volume435
Issue number7043
DOIs
StatePublished - Jun 9 2005
Externally publishedYes

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Symbiosis
Nitrogen
Fungi
Mycelium
Food
Arginine
Carbon
Plant Physiological Phenomena
Embryophyta
Isotope Labeling
Metabolic Networks and Pathways
Ecology
Genes
Minerals
Soil
Gene Expression
Amino Acids

ASJC Scopus subject areas

  • General

Cite this

Govindarajulu, M., Pfeffer, P. E., Jin, H., Abubaker, J., Douds, D. D., Allen, J. W., ... Shachar-Hill, Y. (2005). Nitrogen transfer in the arbuscular mycorrhizal symbiosis. Nature, 435(7043), 819-823. https://doi.org/10.1038/nature03610

Nitrogen transfer in the arbuscular mycorrhizal symbiosis. / Govindarajulu, Manjula; Pfeffer, Philip E.; Jin, Hairu; Abubaker, Jehad; Douds, David D.; Allen, James W.; Bücking, Heike; Lammers, Peter; Shachar-Hill, Yair.

In: Nature, Vol. 435, No. 7043, 09.06.2005, p. 819-823.

Research output: Contribution to journalArticle

Govindarajulu, M, Pfeffer, PE, Jin, H, Abubaker, J, Douds, DD, Allen, JW, Bücking, H, Lammers, P & Shachar-Hill, Y 2005, 'Nitrogen transfer in the arbuscular mycorrhizal symbiosis', Nature, vol. 435, no. 7043, pp. 819-823. https://doi.org/10.1038/nature03610
Govindarajulu M, Pfeffer PE, Jin H, Abubaker J, Douds DD, Allen JW et al. Nitrogen transfer in the arbuscular mycorrhizal symbiosis. Nature. 2005 Jun 9;435(7043):819-823. https://doi.org/10.1038/nature03610
Govindarajulu, Manjula ; Pfeffer, Philip E. ; Jin, Hairu ; Abubaker, Jehad ; Douds, David D. ; Allen, James W. ; Bücking, Heike ; Lammers, Peter ; Shachar-Hill, Yair. / Nitrogen transfer in the arbuscular mycorrhizal symbiosis. In: Nature. 2005 ; Vol. 435, No. 7043. pp. 819-823.
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