Nitric oxide production by chicken macrophages activated by acemannan, a complex carbohydrate extracted from Aloe Vera

K. Karaca, J. M. Sharma, R. Nordgren

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-l-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accouantable for some of the immunomodulatory effects of ACM in chickens.

Original languageEnglish (US)
Pages (from-to)183-188
Number of pages6
JournalInternational Journal of Immunopharmacology
Volume17
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Aloe
Chickens
Nitric Oxide
Macrophages
Carbohydrates
Mannans
Macrophage Activation
Concanavalin A
Nitric Oxide Synthase
Arginine
Spleen
Yeasts
acemannan
Cell Line

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Nitric oxide production by chicken macrophages activated by acemannan, a complex carbohydrate extracted from Aloe Vera. / Karaca, K.; Sharma, J. M.; Nordgren, R.

In: International Journal of Immunopharmacology, Vol. 17, No. 3, 1995, p. 183-188.

Research output: Contribution to journalArticle

@article{279792f99a9f4713a6a8f36f76e0a948,
title = "Nitric oxide production by chicken macrophages activated by acemannan, a complex carbohydrate extracted from Aloe Vera",
abstract = "Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-l-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accouantable for some of the immunomodulatory effects of ACM in chickens.",
author = "K. Karaca and Sharma, {J. M.} and R. Nordgren",
year = "1995",
doi = "10.1016/0192-0561(94)00102-T",
language = "English (US)",
volume = "17",
pages = "183--188",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Nitric oxide production by chicken macrophages activated by acemannan, a complex carbohydrate extracted from Aloe Vera

AU - Karaca, K.

AU - Sharma, J. M.

AU - Nordgren, R.

PY - 1995

Y1 - 1995

N2 - Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-l-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accouantable for some of the immunomodulatory effects of ACM in chickens.

AB - Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-l-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accouantable for some of the immunomodulatory effects of ACM in chickens.

UR - http://www.scopus.com/inward/record.url?scp=0028931679&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028931679&partnerID=8YFLogxK

U2 - 10.1016/0192-0561(94)00102-T

DO - 10.1016/0192-0561(94)00102-T

M3 - Article

VL - 17

SP - 183

EP - 188

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 3

ER -