Nitric oxide inducing factor as a measure of antigen and mitogen-specific T cell responses in chickens

Kemal Karaca, In Jeong Kim, Sudhir K. Reddy, Jagdev M. Sharma

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We describe here an assay to measure responses of T cells to in vitro stimulation with antigens and a T cell mitogen (ConA). Spleen cells from chickens immunized with live viruses and an inactivated antigen produced macrophage activating factors (MAF) in response to in vitro stimulation with homologous antigens. The production of MAF, quantitated by the induction of NO in a retrovirus transformed macrophage cell line, HD11 (Beug et al., 1979, Cell 18, 375) was antigen-specific and correlated well with T cell proliferation. Further studies showed that production of MAF was abrogated by cyclosporin A, anti-CD4 and anti-CD8 monoclonal antibodies. These data suggested that production of MAF required T cell activation and can be used as a measure of antigen and mitogen-specific T cell responses in chickens.

Original languageEnglish (US)
Pages (from-to)97-103
Number of pages7
JournalJournal of Immunological Methods
Volume192
Issue number1-2
DOIs
StatePublished - Jun 10 1996
Externally publishedYes

Fingerprint

Macrophage-Activating Factors
Mitogens
Chickens
Nitric Oxide
T-Lymphocytes
Antigens
Transformed Cell Line
Retroviridae
Cyclosporine
Spleen
Macrophages
Monoclonal Antibodies
Cell Proliferation
Viruses

Keywords

  • chicken
  • nitric oxide inducing factor
  • T cell proliferation

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Nitric oxide inducing factor as a measure of antigen and mitogen-specific T cell responses in chickens. / Karaca, Kemal; Kim, In Jeong; Reddy, Sudhir K.; Sharma, Jagdev M.

In: Journal of Immunological Methods, Vol. 192, No. 1-2, 10.06.1996, p. 97-103.

Research output: Contribution to journalArticle

Karaca, Kemal ; Kim, In Jeong ; Reddy, Sudhir K. ; Sharma, Jagdev M. / Nitric oxide inducing factor as a measure of antigen and mitogen-specific T cell responses in chickens. In: Journal of Immunological Methods. 1996 ; Vol. 192, No. 1-2. pp. 97-103.
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