Nitric oxide inducing factor as a measure of antigen and mitogen-specific T cell responses in chickens

Kemal Karaca, In Jeong Kim, Sudhir K. Reddy, Jagdev M. Sharma

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

We describe here an assay to measure responses of T cells to in vitro stimulation with antigens and a T cell mitogen (ConA). Spleen cells from chickens immunized with live viruses and an inactivated antigen produced macrophage activating factors (MAF) in response to in vitro stimulation with homologous antigens. The production of MAF, quantitated by the induction of NO in a retrovirus transformed macrophage cell line, HD11 (Beug et al., 1979, Cell 18, 375) was antigen-specific and correlated well with T cell proliferation. Further studies showed that production of MAF was abrogated by cyclosporin A, anti-CD4 and anti-CD8 monoclonal antibodies. These data suggested that production of MAF required T cell activation and can be used as a measure of antigen and mitogen-specific T cell responses in chickens.

Original languageEnglish (US)
Pages (from-to)97-103
Number of pages7
JournalJournal of Immunological Methods
Volume192
Issue number1-2
DOIs
StatePublished - Jun 10 1996

Keywords

  • T cell proliferation
  • chicken
  • nitric oxide inducing factor

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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