Nicotinic modulation of gene expression in SH-SY5Y neuroblastoma cells

Travis Dunckley, Ronald J. Lukas

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Exposure to nicotine has a broad range of physiological and psychological effects that can be long lasting and contribute to nicotine dependence. On a time course longer than that needed to activate nicotinic acetylcholine receptor (nAChR) function, nicotine exposure induces functional inactivation of nAChR, upregulation of nAChR radioligand binding sites, and other alterations of cellular functions. To identify possible mechanisms underlying nicotine-induced changes in nAChR numbers and function, we defined changes in gene expression in neuron-like, SH-SY5Y human neuroblastoma cells following 24 h of continuous exposure to 1 mM nicotine. This treatment condition produces both functional inactivation and upregulation of nAChR. Repeat and cross-controlled microarray (∼ 5000 genes queried) analyses revealed 163 genes whose expression was consistently altered at the p < 0.01 level following nicotine treatment. Quantitative, real-time, reverse transcription-polymerase chain reaction analyses confirmed altered expression of thirteen out of fourteen of these genes chosen for further study, including contactin 1, myozenin 2, and ubiquitin-conjugating enzymes E2C and E2S. Inhibition or reversal of these effects by the general nAChR antagonist, d-tubocurarine, indicated that gene expression changes are dependent on nAChR activation. Studies using other nAChR subtype-selective antagonists identified gene expression changes that required activation of both α7- and α3*-nAChR, α7-nAChR alone, or either α7- or α3β4*-nAChR, suggesting some convergent and some divergent pathways of gene activation coupled to these nAChR subtypes. These results suggest that longer-term physiological and psychological effects of nicotine exposure and changes in nAChR expression may be due in part to effects on gene expression initiated by interactions with nAChR.

Original languageEnglish (US)
Pages (from-to)39-49
Number of pages11
JournalBrain Research
Volume1116
Issue number1
DOIs
StatePublished - Oct 20 2006
Externally publishedYes

Fingerprint

Nicotinic Receptors
Neuroblastoma
Gene Expression
Nicotine
Contactin 1
Up-Regulation
Ubiquitin-Conjugating Enzymes
Psychology
Tubocurarine
Tobacco Use Disorder
Cholinergic Antagonists
Transcriptional Activation
Genes
Reverse Transcription

Keywords

  • Gene expression
  • Microarray
  • nAChR
  • Neuroblastoma cells
  • Nicotine
  • SH-SY5Y cells
  • Upregulation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Nicotinic modulation of gene expression in SH-SY5Y neuroblastoma cells. / Dunckley, Travis; Lukas, Ronald J.

In: Brain Research, Vol. 1116, No. 1, 20.10.2006, p. 39-49.

Research output: Contribution to journalArticle

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