Nicotine modulates the expression of a diverse set of genes in the neuronal SH-SY5Y cell line

Travis Dunckley, Ronald J. Lukas

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Nicotine exposure can have long lasting effects on nervous system function, some of which must contribute to nicotine dependence. Up-regulation, an increase in numbers of radioligand-binding nicotinic acetylcholine receptors (nAChR), occurs on exposure to nicotine at high concentrations. To determine whether altered gene expression might account for long term changes and up-regulation following nicotine exposure, we assessed effects of 1 h of 1 mM nicotine exposure on alter. ation of gene expression in the neuron-like SH-SY5Y neuroblastoma clonal line. Repeat and cross-controlled microarray analyses yielded a list of 17 genes from the initially screened ∼5,000 whose expression was consistently altered following nicotine treatment. Subsequent quantitative, real time reverse transcriptase PCR analyses confirmed altered expression in 14 of 16 genes tested. Further, the general nAChR antagonist, d-tubocurarine, blocked all but two of the observed changes in gene expression, indicating that these changes are dependent on nAChR activation. Use of other antagonists revealed that nAChR subtypes can differentially affect gene expression. The genes affected code for proteins that may be broadly categorized into four groups: transcription factors, protein processing factors, RNA-binding proteins, and plasma membrane-associated proteins. Our results suggest that nicotinic activation of nAChR may have a broad role in affecting cellular physiology through modulating gene expression.

Original languageEnglish (US)
Pages (from-to)15633-15640
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOIs
StatePublished - May 2 2003
Externally publishedYes

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Nicotinic Receptors
Nicotine
Gene expression
Genes
Cells
Gene Expression
Cell Line
Up-Regulation
Tubocurarine
Chemical activation
Tobacco Use Disorder
RNA-Binding Proteins
Cholinergic Antagonists
Microarray Analysis
Reverse Transcriptase Polymerase Chain Reaction
Neuroblastoma
RNA-Directed DNA Polymerase
Nervous System
Physiology
Neurology

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nicotine modulates the expression of a diverse set of genes in the neuronal SH-SY5Y cell line. / Dunckley, Travis; Lukas, Ronald J.

In: Journal of Biological Chemistry, Vol. 278, No. 18, 02.05.2003, p. 15633-15640.

Research output: Contribution to journalArticle

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