Nickel-inducible lysis system in Synechocystis sp. PCC 6803

Xinyao Liu, Roy Curtiss

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO4. Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.

Original languageEnglish (US)
Pages (from-to)21550-21554
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number51
DOIs
StatePublished - Dec 22 2009

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Synechocystis
Nickel
Bacteriophages
Biofuels
Genes
Autolysis
Cyanobacteria
Signal Transduction
Genome
Lipids

Keywords

  • Biofuel
  • Phage lysis genes

ASJC Scopus subject areas

  • General

Cite this

Nickel-inducible lysis system in Synechocystis sp. PCC 6803. / Liu, Xinyao; Curtiss, Roy.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 51, 22.12.2009, p. 21550-21554.

Research output: Contribution to journalArticle

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