Next-generation high-density self-assembling functional protein arrays

Niroshan Ramachandran, Jacob V. Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G. Fuentes, Andreas Rolfs, Yanhui Hu, Joshua LaBaer

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

Original languageEnglish (US)
Pages (from-to)535-538
Number of pages4
JournalNature Methods
Volume5
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

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Protein Array Analysis
Complementary DNA
Proteins
Nucleic Acids
Microarrays
Throughput

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Ramachandran, N., Raphael, J. V., Hainsworth, E., Demirkan, G., Fuentes, M. G., Rolfs, A., ... LaBaer, J. (2008). Next-generation high-density self-assembling functional protein arrays. Nature Methods, 5(6), 535-538. https://doi.org/10.1038/nmeth.1210

Next-generation high-density self-assembling functional protein arrays. / Ramachandran, Niroshan; Raphael, Jacob V.; Hainsworth, Eugenie; Demirkan, Gokhan; Fuentes, Manuel G.; Rolfs, Andreas; Hu, Yanhui; LaBaer, Joshua.

In: Nature Methods, Vol. 5, No. 6, 06.2008, p. 535-538.

Research output: Contribution to journalArticle

Ramachandran, N, Raphael, JV, Hainsworth, E, Demirkan, G, Fuentes, MG, Rolfs, A, Hu, Y & LaBaer, J 2008, 'Next-generation high-density self-assembling functional protein arrays', Nature Methods, vol. 5, no. 6, pp. 535-538. https://doi.org/10.1038/nmeth.1210
Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG, Rolfs A et al. Next-generation high-density self-assembling functional protein arrays. Nature Methods. 2008 Jun;5(6):535-538. https://doi.org/10.1038/nmeth.1210
Ramachandran, Niroshan ; Raphael, Jacob V. ; Hainsworth, Eugenie ; Demirkan, Gokhan ; Fuentes, Manuel G. ; Rolfs, Andreas ; Hu, Yanhui ; LaBaer, Joshua. / Next-generation high-density self-assembling functional protein arrays. In: Nature Methods. 2008 ; Vol. 5, No. 6. pp. 535-538.
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