Next-generation high-density self-assembling functional protein arrays

Niroshan Ramachandran, Jacob V. Raphael, Eugenie Hainsworth, Gokhan Demirkan, Manuel G. Fuentes, Andreas Rolfs, Yanhui Hu, Joshua LaBaer

Research output: Contribution to journalArticlepeer-review

252 Scopus citations

Abstract

We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.

Original languageEnglish (US)
Pages (from-to)535-538
Number of pages4
JournalNature Methods
Volume5
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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