New norditerpenoids and a diterpenoid from a sponge that inhibit the lyase activity of DNA polymerase β

V. S.Prakash Chaturvedula, Zhijie Gao, Shannon H. Thomas, Sidney M. Hecht, David G.I. Kingston

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Using an assay to detect inhibitors of the lyase activity of DNA polymerase β, bioassay-directed fractionation of a CHCl 3 extract of an unidentified sponge of the family Demospongiae resulted in the isolation of the new diterpenoid 1, the new bis-norditerpenoids 2-3, and the two known compounds spongia-13(16),14-dien-19-oic acid (4), and methylspongia-13(16),14-dien-19-oate (5). The structures of the new compounds were established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited the lyase activity of DNA polymerase β. Bioassay-directed fractionation of a CHCl 3 extract of an unidentified sponge of the family Demospongiae resulted in the isolation of the new diterpenoid 1, the new bis-norditerpenoids 2-3, and two known compounds. All the isolated compounds were weak inhibitors of the lyase activity of DNA polymerase β.

Original languageEnglish (US)
Pages (from-to)9991-9995
Number of pages5
JournalTetrahedron
Volume60
Issue number44
DOIs
StatePublished - Oct 25 2004
Externally publishedYes

Keywords

  • Bioactivity
  • DNA polymerase β
  • Diterpenoid
  • Marine
  • Sponge

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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