TY - JOUR
T1 - New Insights into the Evolution of the W Chromosome in Lepidoptera
AU - Dalíková, Martina
AU - Zrzavá, Magda
AU - Hladová, Irena
AU - Nguyen, Petr
AU - Šonský, Ivan
AU - Flegrová, Martina
AU - Kubíčková, Svatava
AU - Voleníková, Anna
AU - Kawahara, Akito Y.
AU - Peters, Ralph S.
AU - Marec, František
AU - Sayres, Melissa Wilson
N1 - Funding Information:
This research was funded by grant 14-22765S and follow-up grant 17-13713S of the Czech Science Foundation (CSF). P.N. was supported by CSF grant 14-35819P. I.H. and A.V. thank for further support to the grant 159/2016/P of the Grant Agency of University of South Bohemia. S.K. acknowledges support from The Ministry of Education, Youth, and Sports of the Czech Republic (CEITEC 2020 project LQ1601) and A.K. from the National Science Foundation (DEB #1557007).
Publisher Copyright:
© The American Genetic Association 2017. All rights reserved.
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Moths and butterflies (Lepidoptera) represent the most diverse group of animals with heterogametic females. Although the vast majority of species has a WZ/ZZ (female/male) sex chromosome system, it is generally accepted that the ancestral system was Z/ZZ and the W chromosome has evolved in a common ancestor of Tischeriidae and Ditrysia. However, the lack of data on sex chromosomes in lower Lepidoptera has prevented a formal test of this hypothesis. Here, we performed a detailed analysis of sex chromosomes in Tischeria ekebladella (Tischeriidae) and 3 species representing lower Ditrysia, Cameraria ohridella (Gracillariidae), Plutella xylostella (Plutellidae), and Tineola bisselliella (Tineidae). Using comparative genomic hybridization we show that the first 3 species have well-differentiated W chromosomes, which vary considerably in their molecular composition, whereas T. bisselliella has no W chromosome. Furthermore, our results suggest the presence of neo-sex chromosomes in C. ohridella. For Z chromosomes, we selected 5 genes evenly distributed along the Z chromosome in ditrysian model species and tested their Z-linkage using qPCR. The tested genes (Henna, laminin A, Paramyosin, Tyrosine hydroxylase, and 6-Phosphogluconate dehydrogenase) proved to be Z-linked in all species examined. The conserved synteny of the Z chromosome across Tischeriidae and Ditrysia, along with the W chromosome absence in the lower ditrysian families Psychidae and Tineidae, suggests a possible independent origin of the W chromosomes in these 2 lineages.
AB - Moths and butterflies (Lepidoptera) represent the most diverse group of animals with heterogametic females. Although the vast majority of species has a WZ/ZZ (female/male) sex chromosome system, it is generally accepted that the ancestral system was Z/ZZ and the W chromosome has evolved in a common ancestor of Tischeriidae and Ditrysia. However, the lack of data on sex chromosomes in lower Lepidoptera has prevented a formal test of this hypothesis. Here, we performed a detailed analysis of sex chromosomes in Tischeria ekebladella (Tischeriidae) and 3 species representing lower Ditrysia, Cameraria ohridella (Gracillariidae), Plutella xylostella (Plutellidae), and Tineola bisselliella (Tineidae). Using comparative genomic hybridization we show that the first 3 species have well-differentiated W chromosomes, which vary considerably in their molecular composition, whereas T. bisselliella has no W chromosome. Furthermore, our results suggest the presence of neo-sex chromosomes in C. ohridella. For Z chromosomes, we selected 5 genes evenly distributed along the Z chromosome in ditrysian model species and tested their Z-linkage using qPCR. The tested genes (Henna, laminin A, Paramyosin, Tyrosine hydroxylase, and 6-Phosphogluconate dehydrogenase) proved to be Z-linked in all species examined. The conserved synteny of the Z chromosome across Tischeriidae and Ditrysia, along with the W chromosome absence in the lower ditrysian families Psychidae and Tineidae, suggests a possible independent origin of the W chromosomes in these 2 lineages.
KW - Comparative genomic hybridization
KW - Laser microdissection
KW - Quantitative PCR
KW - Sex chromosome evolution
KW - Synteny mapping
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U2 - 10.1093/jhered/esx063
DO - 10.1093/jhered/esx063
M3 - Article
C2 - 28992287
AN - SCOPUS:85032736401
SN - 0022-1503
VL - 108
SP - 709
EP - 719
JO - Journal of Heredity
JF - Journal of Heredity
IS - 7
ER -