New bimetallic porphyrin film: An electrocatalytic transducer for hydrogen peroxide reduction, applicable to first-generation oxidase-based biosensors

Julio Miguel Vago, Viviana Campo Dall'Orto, Erica Forzani, Jorge Hurst, Irene Noemí Rezzano

Research output: Contribution to journalArticle

18 Scopus citations


A bimetallic porphyrin film was studied as electrode modifier with catalytic activity for molecular oxygen reduction and hydrogen peroxide reduction. Molecular oxygen reduction is catalyzed by both polyFePPIX or polyCuPPIX. Peroxidase activity at-200 mV versus Ag/AgCl is only performed by polyFePPIX in absence of oxidative agents, and the deposit polyCuPPIX is required to perform peroxidase activity in the presence of oxygen. Copper substitution by other metals in the porphyrin was not effective. It has been found that metalloporphyrin immobilization order acquire particular relevance. However, the mass of porphyrin deposit is always 1.5-1.6 μg of polyFePPIX and 7.3-9.1 μg of polyCuPPIX, indicating that polymer growth rate is not affected by immobilization order. polyCuPPIX deposited on polyFePPIX preserves catalytic properties over a wide pH range, and acts as a molecular sieve. The use of this bimetallic electrode in a first-generation oxidase biosensors is discussed.

Original languageEnglish (US)
Pages (from-to)407-412
Number of pages6
JournalSensors and Actuators, B: Chemical
Issue number1-2
StatePublished - Nov 15 2003
Externally publishedYes



  • Bimetallic film
  • Catalyst
  • Electropolymerization
  • Hydrogen peroxide

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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